摘要
目的探讨γ-氨基丁酸(GABA)对情绪应激大鼠血脂代谢的调节作用。方法利用不确定性空瓶刺激的方法建立焦虑应激大鼠模型,将40只雄性SD大鼠随机分为对照组、模型组、GABA 0.5(L)、1.0(M)和2.0 mg/kg(H)组,应激前分别给予大鼠灌服生理盐水或相应剂量的GABA,14 d后采用高架十字迷宫实验(EPM)检测大鼠行为,然后采集血样,测定大鼠血浆甘油三酯(TG)、总胆固醇(TC)、低密度脂蛋白胆固醇(LDL-C)和高密度脂蛋白胆固醇(HDL-C)水平,并计算非高密度脂蛋白胆固醇(non-HDL-C)含量、TG/HDL-C、LDL-C/HDL-C比值。结果:应激后对照组体重和食物利用率极显著高于其余各组(P<0.01);模型组进入开放臂的时间百分比极显著低于对照组和GABA 2 mg/kg组(P<0.01),进入开放臂的次数百分比则显著低于对照组(P<0.05);各组大鼠的入臂总次数差异不显著(P>0.05)。血脂水平分析显示模型组血浆中TC含量显著高于GABA 2 mg/kg组(P<0.05),non-HDL-C含量则显著高于对照组(P<0.05),极显著高于GABA 2 mg/kg组(P<0.01),模型组的LDL-C/HDL-C比值显著高于对照组和GABA 2 mg/kg组(P<0.05)。结论 2 mg/kg GABA能明显增加EPM中进入开放臂的时间百分比,降低TC与non-HDL-C含量并下调LDL-C/HDL-C比值,有效缓解焦虑情绪并改善血脂代谢。
Objective To observe the modulation effects of Y-aminobutyric acid （GABA） on blood lipids in rats under emotional stress. Methods Forty male Sprague-Dawley rats were divided into 5 groups randomly （n=8 each）： control group, model group, GABA 0.5, 1.0 and 2.0 mg/kg group. Animal model of emotional stress was induced by empty water bottles stimulation method. The rats were administered with saline or different doses of GABA orally before they suffered from emotional stress. 14 d later, animal behaviors were assessed in the elevated plus maze test （EPM）, and plasma levels of triglyceride （TG）, total cholesterol （TC）, low density lipoprotein-cholesterol （LDL-C） and high density lipoprotein- cholesterol （HDL-C） were determined. Non-high density lipoprotein-cholesterol （non-HDL-C）, TG/HDL-C and LDL- C/HDL-C ratio were also calculated. Results After 14 d-emotional stress procedure, the body weight and food utilization rate of control group were higher than those of other groups significantly （P〈0.01）. Model group had a decreased percentage of time spent in open arms than control and GABA 2 mg/kg group in the EPM test （P〈0.01）. Meanwhile, the percentage of entrance frequency to open arms of model group was lower than control group （P〈0.05）, and the total entrance was not significantly different among different groups （P〈0.05）. The analysis of blood lipids showed that higher concentration of TC was found in model group, as compared with GABA 2 mg/kg group （P〈0.05）. The levels of non-HDL-C in model group was significantly higher than those in control group （P〈0.05） and GABA 2 mg/kg group （P〈0.01） respectively. Moreover, LDL-C/HDL-C ratio of model group was significantly higher than control and GABA 2 mg/kg group （P〈 0.05）. Conclusion Administration of 2 mg/kg GABA may relieve anxiety and improve blood lipids by increasing the percentage of time spent in open arms in EPM and reducing plasma TC, non-HDL-C levels and LDL-C/HDL-C ratio. [ACTA NUT
出处
《营养学报》
CAS
CSCD
北大核心
2013年第3期241-245,共5页
Acta Nutrimenta Sinica
基金
国家荔枝龙眼产业技术体系(No.CARS-33)
关键词
Γ-氨基丁酸
焦虑
血脂
情绪应激
高架十字迷宫实验
Y-aminobutyric acid
anxiety
blood lipids
emotional stress
elevated plus maze test
