摘要
目的探讨T淋巴细胞亚群在面部激素依赖性皮炎发病机制中的作用。方法应用免疫组织化学SP法检测25例面部激素依赖性皮炎患者皮损、10例面部亚急性湿疹患者皮损、10例外科手术切取的面部外观正常皮肤T淋巴细胞亚群分布特点。结果正常皮肤组织CD4+,CD8+T细胞在真皮浅层呈阴性或少量表达,面部亚急性湿疹皮损和面部激素依赖性皮炎皮损中CD4+,CD8+T细胞在真皮乳头、真皮浅层T淋巴细胞胞膜上有大量表达。面部激素依赖性皮炎组CD8+T细胞数(219.24±125.62)显著高于对照组(36.10±22.10),差异具有统计学意义(P<0.017);面部亚急性湿疹组皮损中CD8+T细胞数(153.70±112.42)明显高于对照组,差异具有统计学意义(P<0.017);面部激素依赖性皮炎组和面部亚急性湿疹组皮损中CD4+T细胞数与对照组相比有所增加,但三组间差异无统计学意义(P>0.05)。面部激素依赖性皮炎皮损中CD4+/CD8+比值升高,但三组间差异无统计学意义(P>0.05)。结论 T淋巴细胞亚群的变化可能参与了面部激素依赖性皮炎的免疫病理过程。
Objective To observe the expression of T lymphocyte subsets in lesions of facial steroid-dependent dermatitis and to explore the pathogenesis. Methods Twenty-five patients with facial steroid-dependent dermatitis and ten patients with facial subacute eczema were diagnosed clinically. Ten healthy skin specimens were taken as controls. The distribution and intensity of expression of T lymphocyte subsets in the specimens were detected by immunohistochemieal SP method. Results There was not or little CD4 ＋ and CD8 ＋ T were detec- ted cell in the superficial dermis of normal skin tissue, but a large number of CD4＋ and CD8 ＋ T cell were expressed at the dermal papilla, superficial dermis in facial subacute eczema and facial steroid-dependent dermatitis. The level of CD8 ＋ T cell in facial steroid-dependent dermatitis were higher than that of controls, and there had a significant statistical difference （P 〈 0.017） ; The level of CD8 ＋ T cell in facial subacute eczema were higher than that of controls, and there had a significant statistical difference（P 〈 0.017 ）. The level of CD4 ＋ T cell in facial steroid-dependent dermatitis and facial subacute eczema were higher than that of controis, but they hadn＇t statistically significant difference （ P 〉 0.05 ）. Although the CD4 ＋/CD8 ＋ ratio in facial steroid-dependent dermatitis increased, compared with controls, there was no statistical difference （ P 〉 0.05）. Conclusion The change of T lymphocyte subsets in the lesion of facial steroid dependence dermatitis, which maybe participated in immunopathogenesis of facial steroid dependence dermatitis.
出处
《中国皮肤性病学杂志》
CAS
北大核心
2013年第7期665-666,676,共3页
The Chinese Journal of Dermatovenereology
