摘要
用Ｉｍｏｋａｗａ等的实验方法，用１０种化妆品及８种化妆品化学成分涂于黄棕色豚鼠侧背部皮肤，然后取材，进行ＨＥ染色、黑素染色及Ｄｏｐａ氧化酶染色，以探讨化妆品及其化学成分的致色素作用。结果：１０种化妆品均显著增加黄棕色豚鼠表皮基底细胞中含黑素的细胞数，而８种化妆品成分中仅胭脂红、对羟基苯甲酸、柠檬黄、松香、羊毛脂等５种引起此种改变。提示此动物模型易于显示化妆品的致色素作用，又可显示化学品致色素作用的差异。并观察到组织有无炎症反应及反应程度与色素沉着不完全平行，提示化妆品及其成分所致的色素沉着并非全是炎症的后果，因此，在化妆品生产中不仅要避免化妆品中所含的致敏物质。
Pigmentedcosmeticdermatitiscomposesamajorsortofundesirablereactionsinducedbycosmetics,butthemelanogenicefectofcosmeticscanscarcelybedetectedwithpatchtestsinhuman.Inthepresentstudy,10cosmeticproductsmostfrequentlyusedbythepatientswithpigmentedcosmet-icdermatitisand8ingredientscontainedinthecosmeticswerepaintedontoseparateareasofthedorsalflankofbrownishguineapigsfolowingthemethodreportedbyImokawaetal,thenskinspecimenswereremoved,theparafinimbededsectionswerestainedwithH.E.,Schmorl′sreactionwasusedtoshowmelanin,andBacher,PraverandThatcher′sdopa-oxidasereactiontostudythemelanogenicef-fectofthecosmeticsandtheingredients.Theresultsshowedthatalofthe10cosmeticstestedsignifi-cantlyincreasedthenumberofcelscontainingmelaninintheepidermalbasallayeroftheguineapigs,whileamongthe8ingredientstested,only5(carmine,parabens,limocitrin,resinandlanolin)re-vealedthiskindofefect.Thissuggeststhattheguineapigmodel,beingsensitiveinresponsetothemelanogenicactivityofcosmeticsontheonehandandreactingdiferentialytovariouskindsofingredi-entsontheother,mightbeusedinassayingthemelanogeniceffectofcosmeticproducts.Theexistenceandthedegreeofthehistologicalinflammatoryreactioninducedbythetestedmaterialsdidnotgoparal-leltothoseofhyperpigmentation,suggestingthatcosmeticinducinghyperpigmentationmaynotbesim-plyasequenceofdermatitis.Therefore,tosolvetheproblemofcosmetichyperpigmentationitshouldnotonlyavoidthesensitizers,butalsothemelanogenicagentscontainedinthecosmeticproducts.
出处
《中华皮肤科杂志》
CAS
CSCD
北大核心
1996年第6期430-432,共3页
Chinese Journal of Dermatology
关键词
化妆品
色素沉着
不良反应
MelanogensisCosmeticsGuineapig,brownish
