摘要
目的探讨钙释放激活钙离子通道(calcium release-activated calcium,CRAC)关键蛋白STIM1在幼年和成年大鼠外伤性癫痫中的表达差异及意义。方法取SD雄性幼年、成年大鼠各94只,按照完全随机设计分为成年、幼年假手术组:皮层注射生理盐水,各42只;成年、幼年模型组:铁离子皮层注射外伤性癫痫模型,各52只。建模后观察大鼠癫痫行为学表现;分别于6、24、72 h,7、14、21、28 d 7个时相点完全随机选取6只大鼠,处死取伤灶周边皮层脑组织,用实时荧光定量PCR、Western blot和免疫组织化学法分别检测STIM1的mRNA与蛋白的表达。结果模型组成年、幼年大鼠均出现典型痫性发作,幼鼠模型组建模成功率为91.3%,成鼠模型组建模成功率为84%(定义Racine评分3分及以上为建模成功)。幼鼠模型组比成鼠模型组发作次数明显增多(P<0.05),每次发作持续时间显著延长(P<0.05),发作程度较重。建模后各组STIM1的mRNA和蛋白表达都有明显增高,72 h达高峰(P<0.05),但幼鼠与成鼠相比较,STIM1的mRNA和蛋白在各时间点表达增高的趋势更加显著(P<0.05)。结论幼鼠较成鼠更容易发生外伤性癫痫,STIM1在幼鼠中较成鼠的表达也更高,提示STIM1表达增加、CRAC激活可能是幼鼠更易发生外伤性癫痫的机制之一。
Objective To detect the differential expression of STIM1, a key molecule in calcium re- lease-activated calcium （CRAC）, and investigate its significance between young and adult rats with posttraumatic epilepsy. Methods SD male adult and young rats （n = 94 for each age group） were randomized into sham- operation group （injected by normal saline into the cortex, n = 42 for each age group） and model group （injec- ted by FeC13 into the cortex, n = 52 for each age group）. The behavioral changes of the sham-operation and model groups were carefully observed and evaluated by modified Racine stage. In 6, 24 and 72 h, and 7, 14, 21 and 28 d after the injection, the rats of different age groups were sacrificed for the cortex tissue around the injection site, with 6 rats randomly selected at each time point. Real-time fluorescence quantitative PCR （RT-PCR）, Western blotting and immunohistochemical assay were used to detect the expression of STIM1 at mRNA and protein levels. Results Epileptic seizure was found only in the model group of adult and young rats. In the models with epilepsy induced by FeC13, the achievement rate was 91.3% in the young rats, but only 84% in the adult rats （the definition of the successful model was rats with Racine score above 3 ）. Compared with the model group of adult rats, the model group of young rats had significantly more epileptic sei- zures （P 〈 0. 05）, and also had significantly stronger severity and longer duration of epileptic seizure （ P 〈 0. 05 ）. The expression of STIM1 at mRNA and protein levels was significantly stronger in the model group than in the sham-operation group （P 〈 0. 05 ） in each time point, and reached the highest level in 72 h （ P 〈 0. 05 ）, with that in the young rats significantly higher than in adult group at each time point. Conclusion Young rats are prone to posttraumatic epileptic seizures, and severer than adult rats. The expression of STIM1 is higher in young rats than in adult rats, suggesting that higher expre
出处
《第三军医大学学报》
CAS
CSCD
北大核心
2013年第14期1493-1497,共5页
Journal of Third Military Medical University
基金
国家临床重点专科建设项目经费资助([2011]170)
重庆市卫生局课题(2010-2-002)~~
关键词
外伤性癫痫
CRAC通道
STIM1
posttraumatic epilepsy
calcium release-activated calcium channel
STIM1
