摘要
目的探讨黄体酮对蛛网膜下腔出血(SAH)后早期脑损伤(EBI)中细胞凋亡、血脑屏障(BBB)稳定性、脑水肿和死亡率方面的保护作用。方法 66只大鼠被随机分为假手术组、SAH+溶剂组、SAH+黄体酮组。于SAH模型制成后1h、6h和12h分别给予黄体酮(16毫克/千克体重)或等体积的溶剂。在SAH后24h分析不同组间大鼠在死亡率、神经功能评分、脑水肿、细胞凋亡、caspase-3及基质金属蛋白酶-9(MMP-9)表达水平的差异。结果与SAH+溶剂组比较,黄体酮治疗显著降低了大鼠的死亡率、细胞凋亡程度以及caspase-3水平与MMP-9的表达水平,减轻了脑水肿和伊文思蓝的渗出,提高了神经功能评分。结论黄体酮可通过抑制细胞凋亡和稳定BBB减轻SAH后EBI。
Objective Toexplore the effect of progesterone on cell apoptosis,stability of the blood-brain barrier（BBB）,brain edema,and mortality in male Sprague-Dawley rats subjected to subarachnoid hemorrhage（SAH）-induced early brain injury（EBI） by endovascular perforation.Methods Rats（n=66） were randomly assigned to sham,SAH＋vehicle,and SAH＋progesterone groups.Progesterone（16mg/kg） or an equal volume of vehicle was administered at 1h,6h and 12h after SAH.Mortality within 24h,neurological scores,brain edema,Evans blue dye extravasation,cell apoptosis,and the expression of caspase-3 and matrix metalloproteinase MMP-9 were assayed after 24h of SAH.Results Progesterone treatment significantly reduced mortality,brain edema,Evans blue dye extravasation,cell apoptosis,expression of caspase-3 and MMP-9,and improved neurologicalscores compared with the vehicle group.Conclusion Progesterone may reduce EBI after SAH by inhibiting cell apoptosis and stabilizing the BBB.
出处
《中风与神经疾病杂志》
CAS
CSCD
北大核心
2013年第6期500-503,共4页
Journal of Apoplexy and Nervous Diseases
基金
卫生厅省部共建项目(No.WSJ2009-2-025)
