摘要
目的研究黄体酮对脑梗死体积及行为学的影响，评价其对脑梗死大鼠的治疗价值。方法按随机数字表法将健康成年雄性Sprague—Dawley大鼠分为4组，即假手术组、模型组、溶剂治疗组和黄体酮治疗组。后3组采用线栓法制作大脑中动脉栓塞大鼠模型，假手术组只分离暴露血管、不结扎动脉、不插入尼龙鱼线；黄体酮治疗组造模成功后腹腔注射黄体酮，溶剂治疗组给予等体积的环糊精溶液治疗。分别于脑梗死后1d、2d、3d用Zea—Longer评分评价大鼠神经功能的缺损程度，并于脑梗死后3d通过TTC染色检测脑梗死体积。结果Zea—Longer评分显示，造模前所有大鼠均没有神经功能缺损症状，评分为0。成模后，除假手术组外，其余大鼠均有不同程度的神经功能缺损；黄体酮治疗组大鼠[（3．00±0．63）分，（2．83±0．75）分，（2．00＋0．89）分]在脑梗死后1d、2d、3d神经功能评分低于模型组[（4．00＋0．89）分，（3．83＋0．75）分，（3．16＋0．75）分]及溶剂治疗组[（3．674-1．21）分，（3．50±1．05）分，（2．83±0．76）分]，差异有显著性（P〈0．05）。TTc染色表明，脑梗死体积在模型组[（23．74＋4．48）％]与溶剂治疗组[（24．42±7．07）％]均比较大，且二者比较差异无统计学意义（P〉0．05）；经黄体酮治疗后[（15．03＋3．75）％]脑梗死体积明显缩小，较模型组与溶剂治疗组差异均有显著性（P〈0．05）。结论黄体酮治疗可以显著减小脑梗死体积并明显促进脑梗死大鼠神经功能的恢复，其对脑梗死大鼠动物模型具有很好的治疗价值。
Objective To study the influence of progesterone （PROG） on infarct volume and functional outcome and to evaluate the therapeutic value of PROG on cerebral infarction in rats. Methods Health adult male Sprague-Dawley rats were randomly divided into sham-operated （control） group, ischemic group, vehicle-treated group and PROG-treated group. Permanent cerebral ischemia was induced by occlusion of the left middle cerebral artery （MCA） using an intraluminal filament technique. Sham-operated rats were subjected to the same surgical procedure, except that the filament was not advanced to occlude the MCA. Progesterone or 2-hydroxypropyl-13-cy- clodextrin was injected intraperitoneally following permanent middle cerebral artery occlusion （PMCAO） of rats. Zea Longa test was used to evaluate their functional outcome at ld,2d,3d after stroke. T＇FC staining was used to detect the infarct volume at 3d after stroke. Results The results of Zea Longa test showed that there were no functional deficits in all animals prior to ischemia. There were no significant changes in motor function in sham-op- erated animals across the 3 days assessment period. Both PROG and vehicle-treated rats experienced significant decline in scores following occlusion. However, PROG-treated rats （ 3.00 ± 0.63,2.83 ± 0.75,2.00 ± 0.89 ） demonstrated a gradual improvement in scores compared with ischemic （4.00 ± 0.89,3.83 ± 0.75,3.16 ± 0.75 ） and vehicle-treated rats （3.67 ± 1.21,3.50 ± 1.05,2.83 ±0.76） at different times （P〈0.05）. TFC staining re- vealed that PROG administration significantly reduced the total infarct volume in the PROG-treated rats （ （ 15.03 ± 3.75 ） % ） compared with ischemic （ （23.74 ± 4.48 ） % ） and vehicle-treated rats （ （24.42 ± 7.07 ） %, P 〈 0.05 ）. Conclusions PROG significantly reduces infarct volume and promotes the recovery of neurological functions after pMCAO, which has good therapeutic value for the rat model of cerebral infarction.
出处
《中华行为医学与脑科学杂志》
CAS
CSCD
北大核心
2011年第12期1092-1094,共3页
Chinese Journal of Behavioral Medicine and Brain Science
基金
河南省卫生厅重大科技攻关项目（WKJ2010-2-016）
关键词
脑梗死
黄体酮
脑梗死体积
神经功能
大鼠
Cerebral infarction
Progesterone
Infarct volume
Neurological functions
Rat
