摘要
本文报道了用本室建立的2株抗人体骨肉瘤细胞株所分泌的单克隆抗体,采用ABC酶标法,对24种肿瘤组织和正常人体组织进行了免疫组化定位研究,发现OS-McAb_1和OS-McAb_2除对部分骨源性恶性肿瘤有阳性反应外,对其他部位的肿瘤均显示阴性反应;与所标记的正常成人及胎儿相应组织无交叉反应。提示OS-McAb对骨肉瘤有较高的特异性,在骨源性恶性肿瘤的免疫诊断方面有一定应用价值。
Immunohistochemical localization of many sorts of tumor tissues from paraffin sections was studied using the ABC method with the monoclonal antibody secreted by the established two anti-osteosarcoma cell lines. It showed that the OS-McAb1 and OS-McAb2 had a negative reaction on the tested benign tumors and malignant tumors of epithelial tissue. Of the tested malignant tumors of mesenchyma, the antibodies had a positive reaction on some osteogenic sarcoma. They did not cross-react with various normal adult or fetal tissues, indicating that the OS-McAbs had a rather high specificity. They had a certain practical value in the immunopathologic diagnosis of malignant osteogenic tumors.
出处
《第二军医大学学报》
CAS
CSCD
北大核心
1991年第5期445-448,共4页
Academic Journal of Second Military Medical University
关键词
骨肉瘤
单克隆抗体
ABC法
osteosarcoma
monoclonal antibody
ABC immunohistochemical method
