摘要
目的通过制备急性肝功能不全的小鼠模型,观察急性肝功能不全小鼠的一般状况及造模后肝脏的病理变化,造模后腹腔注射不同pH值的氯化铵溶液,观察小鼠的肝性脑病的临床表现。方法给予SD小鼠0.4%四氯化碳花生油溶液混悬液(10 mL/kg)腹腔注射联合灌胃给药造模,再腹腔注射给予不同pH值0.3mol/L的氯化铵溶液,并观察小鼠的肝性脑病的临床表现,后取肝固定做病理切片。结果随着造模时间的增加,与对照组比较,实验组小鼠后期有小鼠死亡3只,病理显示实验组小鼠肝脏损伤严重,对氨的耐受性显著下降。结论四氯化碳可造成小鼠急性肝功能不全,且急性肝功能不全小鼠对氨的耐受性随着氯化铵溶液pH值的升高而降低,肝功能不全的临床表现的更严重。
Objective To prepare mouse model with acute hepatic dysfunction, to observe pathological changes of liver after modeling as well as observe clinical manifestations when the mice received intraperitoneal injection of ammonium chloride solution with different pH value. Methods By giving 0. 4% CC14 peanut oil solution suspension （10mL/kg） to SD mice by intraperitoneal injection and gastric lavage combined with medication to make the acute hepatic dysfunction model, then offering ammonium chloride solution with different pH value （0. 3moL/L） by intraperitoneal injection to mice, clinical manifestations were observed in mice with hepatic encephalopathy, and pathological section was made by hepatopexy. Results Three mice died in the experiment group with the increase of modeling time compared with the control group. The pathology suggested that their livers were seriously injured and the tolerance of ammonia was decreased. Conclusion Carbon tetracbloride can cause acute liver dysfunction, and mice with acute liver dysfunction are less tolerant to ammonia with the rise of pH value of ammonium chloride solution, and with more severe clinical manifestation of liver dysfunction.
出处
《辽宁医学院学报》
CAS
2013年第4期18-20,I0002,共4页
Journal of Liaoning Medical University (LNMU) Bimonthly
基金
莆田学院大学生2011-2012科研立项
编号:11366
关键词
急性肝功能不全
氨的耐受性
肝性脑病
acute hepatic dysfunction
ammonia tolerance
hepatic encephalopathy
