摘要
目的通过硫代乙酰胺(TAA,300 mg·kg-1·d-1)不同用药时间诱导A型肝性脑病,比较大鼠的行为学、生物化学以及组织学改变,探讨造模最适时间。方法将大鼠分为A、B、C、D四组,其中A组为正常对照组;B、C、D三组用TAA(300 mg·kg-1·d-1)分别连续灌胃2 d、3 d、4 d,A组用相等量生理盐水灌胃4 d。比较各组大鼠行为学变化、脑功能评分、AHE的诱导率和致死率,并分析各组给药结束24 h后血氨、ALT、AST、TBIL的差异。结果 C、D组比B组大鼠脑功能评分高,差异有统计学意义(P<0.0083);C、D组比B组诱导率高(P<0.0083),而D组比B、C组的大鼠致死率高,差异有统计学意义(P<0.0083);C、D组血氨及ALT、AST、TBIL肝功能指标比B组高,差异有统计学意义(P<0.0083);C、D组TAA用药后肝组织学观察炎症浸润、坏死、纤维化等损害最明显。结论 300 mg·kg-1·d-1的TAA连续灌胃3d,行为学改变显著,致死率较低,血氨较高,肝功能损害明显,为TAA诱导大鼠急性肝性脑病适宜时间。
Objective To explore the appropriate medication time of inducing acute hepatic en- cephalopathy of rats by different medication time of thioacetamide （TAA, 300 mg·kg^-1·d^-1） to change behav- ioral, biochemical and histological status. Methods The animals were categorized in 4 groups（A, B, C, D）and A group was normal control group. Gastric lavage was performed with TAA （300 mg·kg^-1·d^-1） for two days, three days and four days in group B, C, D respectively. Control group was treated with equal amount of physio- logical saline for four days. The behavior changes, brain function score, AHE induced morbidity and mortality were compared, and the differences of ammonia, ALT, AST, TBIL in each group were analyzed 24 hours after the end of administration. Result The grading scores of brain function in C and D groups were higher than in B group （P 〈 0.0083）. The induction rate of C and D groups was higher than that of B group （P 〈 0.0083）, while the death rate of D group was significantly higher than B, C groups （P 〈 0.0083）. The level of ammonia, ALT, AST, TBIL in C and D groups was higher than in B group （P 〈 0.0083）. Histological findings showed that C and D groups were better to induce inflammation, necrosis and fibrosis in the liver of AHE animals. Conclu- sion Gastric gavage with TAA（300 mg·kg^-1·d^-1） for 3 days was the appropriate medication time to induce AHE.
出处
《现代消化及介入诊疗》
2013年第6期344-348,共5页
Modern Interventional Diagnosis and Treatment in Gastroenterology
基金
广州市卫生局(2009B05)
关键词
肝性脑病
急性肝性脑病
硫代乙酰胺
大鼠
Hepatic encephalopathy
Acute hepatic encephalopathy
Thioacetamide
Rat
