摘要
利用同源克隆及RACE方法,从白背飞虱Sogatella furcifera中克隆获得海藻糖酶基因(Treh-2)全长cDNA序列(GenBank登录号:JX204291),该基因全长为2 065 bp,包含73 bp 3,UTR和150 bp5,UTR,开放阅读框(ORF)为1 842 bp,编码613个氨基酸。该基因预测蛋白分子质量为70.45 ku,等电点为5.65,有5个预测糖基化位点,有1个信号肽结构。进化分析结果显示,白背飞虱海藻糖酶Treh-2与其他昆虫海藻糖酶Treh-2同源性较高,与灰飞虱相比,同源性为95%,与褐飞虱相比较,同源性为93%。海藻糖酶Treh-2基因克隆和比较分析为进一步深入研究白背飞虱迁飞能力与能量代谢具有重要意义。
The trehalase gene （Treh-2） from Sogatella furcifera was cloned by homology cloning strategy and technique of Rapid-Amplification of cDNA Ends （RACE）. The full-length cDNA of S. furcifera Treh-2 （Sffreh-2） is 2 065 bp （GenBank number. JX204291） and has an open reading frame of 1 842 bp, a 3, UTR （untranslated region） of 73 bp and a 5, UTR of 150 bp. The ORF of StTreh-2 encodes a polypeptide of 613 amino acids with a calculated molecular weight of 70.45 ku, and pl of 5.65. There are five predicted N-glycosylation sites and one signature peptide. A phylogenetic tree results using BLAST showed that the amino acid sequence of SfTreh-2 has the greatest similarity to other insects. Compared with Laodelphax striatellus and Nilaparvata lugens, they are 95% and 93% identitied, respectively. Cloning and sequence analysis of Treh-2 gene from S. furcifera could be useful and meaningful in the studies of flight ability and energy metabolism for this insect.
出处
《东北农业大学学报》
CAS
CSCD
北大核心
2013年第6期112-117,共6页
Journal of Northeast Agricultural University
基金
遵义师范学院博士科研基金(2012BSJJ06)
