摘要
[目的]体外观察不同浓度的人工合成抗菌肽(artificial antimicrobial peptides,AMPs)对口腔肿瘤细胞系的增殖、侵袭抑制及促凋亡作用。[方法]合成AMPs并配置溶液,将AMPs溶液(50μg/ml^200ug/ml)加至肿瘤细胞培养体系中,收获培养后细胞以流式细胞分析法(Annexin-V/PI染色)进行细胞凋亡检测,并通过BrdU掺入实验以及MTS法进行细胞增殖检测。通过细胞迁移以及侵袭实验评价AMPs对口腔肿瘤细胞系迁移及侵袭能力的影响。[结果]AMPs可诱导口腔肿瘤细胞系SACC-83及Tca8113凋亡。在AMPs 200μmol/L浓度,中晚期凋亡比率分别为(33.89±16.74)%、(32.47±13.53)%,高于PBS对照组的中晚期凋亡比率(4.34±1.08)%和(5.76±1.43)%(P均<0.05),同时AMPs对肿瘤细胞促凋亡作用的效果与其浓度呈剂量依赖特点。BrdU掺入实验及MTS法发现AMPs可显著性抑制口腔肿瘤细胞系SACC-83及Tca8113的细胞增殖,当AMPs浓度为50μmol/L时对人口腔肿瘤细胞系SACC-83和Tca8113细胞增殖活性有明显的抑制作用,AMPs对人口腔肿瘤细胞系SACC-83和Tca8113细胞增殖抑制作用的IC50分别为60.38μM和55.35μM。低浓度(50μmol/L)AMPs还可显著性抑制SACC-83和Tca8113细胞的迁移以及侵袭。[结论 ]AMPs体外对口腔肿瘤细胞系SACC-83及Tca8113有明显的促进凋亡作用,并抑制肿瘤细胞的增殖、迁移及侵袭。
[Purposel To investigate the effect of artificial antimicrobial peptides （AMPs） on prolifera- tion,invasion,metastasis and apoptosis in oral tumor cell line in vitro. [Methods] Synthesis AMPs and dissolving the AMPs into PBS. The solution of AMPs （50μg/ml-200ug/ml） was added to the tumor cell culture system. The tumor cells apoptosis was detected by flow cytometry analysis method （Annexin- V/PI staining） ,and proliferation was examined by BrdU and MTS. Transwell migration assay and inva- sion assay were used to assess the ability of oral tumor cells migration and invasion treated by AMPs. [Resultsl The AMPs could induce the oral tumor cells apoptosis. In the concentration of AMPs 200μmol/L,the percent of late stage apoptosis in oral tumor cells SACC-83 and Tca8113 were （33.89±16.74）% and （32.47±13.53）% respectively,which was significantly higher than those in PBS control group [（4.34±1.08）% and （5.76±1.43）%]（P〈0.05）. While the effect of AMPs on inducing apoptosis of tumor cells showed dose-effect with the AMPs concentration. Evaluating by BrdU incorporation assay and the MTS proliferation assay,AMPs also inhibited the proliferation in oral tumor cell lines（SACC-83 and Tca8113）. When the AMPs concentration of 50μmol/L,the human oral tumor cell lines SACC-83 Tea8113 cell proliferation was inhibited significantly. The cell proliferation IC50 of AMPs on human oral cancer cell lines SACC-83 and Tca8113 were 60.38μM and 55.35μ M respectively. The low concentra- tion of AMPs （50μmol/L） also significantly inhibited the migration and invasion in SACC-83 and Tca8113 cell. [Conclusions] The AMPs can significantly induce the apoptosis , and effectively inhibit the proliferation,invasion and metastasis of oral tumor cell lines SACC-83 and Tca8113 in vitro.
出处
《中国肿瘤》
CAS
2013年第6期466-472,共7页
China Cancer
基金
北京市自然科学基金资助项目(7123214)
关键词
人工合成抗菌肽
口腔肿瘤细胞系
细胞凋亡
细胞增殖
迁移
侵袭
artificial antimicrobial peptides
oral tumor cell lines
proliferation
apoptosis
invasion
metastasis
