摘要
采用暴露实验方法,研究青、土霉素混合废水出水不同暴露浓度对锦鲤肝脏组织中超氧化物歧化酶(SOD)和过氧化氢酶(CAT)活性、丙二醛(MDA)含量的影响。结果显示:青、土霉素混合废水出水对锦鲤肝脏SOD活性变化影响不大,只在1/16φ(96 h LC50)浓度组8 d时才出现显著诱导。1/16、1/256φ(96 h LC50)浓度组CAT活性呈相似的"上升-下降-上升"的周期性波动规律,1/4φ(96 h LC50)浓度组CAT活性表现出双峰型变化趋势,峰值出现在4 d和8 d。1/256φ(96 h LC50)浓度组MDA含量呈现出单峰型变化趋势,峰值出现在6 d;1/16、1/4φ(96 h LC50)浓度组MDA含量随暴露时间的延长呈现"上升-平缓-上升"的趋势,暴露初期(2 d)和暴露末期(10 d),各浓度组MDA含量表现出一定的剂量效应关系,暴露浓度越大,机体受到的氧化损伤越明显。暴露实验结果表明,CAT活性产生较大变化,引起了鱼体产生较强的氧化压力,鱼体肝脏组织出现不同程度的氧化损伤,当鱼体内的抗生素浓度达到一定程度时,机体氧化损伤能够得到明显减轻,当机体中土霉素浓度超出了其清除自由基的范围时,机体再次出现明显的氧化损伤;锦鲤肝脏中CAT和MDA对青、土霉素混合废水出水暴露都较为敏感,能够较好地反映氧化胁迫的情况,适合作为水环境中青、土霉素污染监测的水生生物指标。
An exposure experiment was made to study the effect of different concentrations of mixed wastewater effluent of penicillin and oxytetracycline on the activities of superoxide dismutase(SOD),catalase(CAT),and malondialdehyde(MDA) content in liver tissue of Cyprinus carpio.The results showed that mixed wastewater effluent of penicillin and oxytetracyline had little effect on SOD activity,significant induction only occurred in 1 /16 φ(96 h LC 50) group on the eighth day.The CAT activity of the 1/16 φ(96 h LC 50) and the 1 /256 φ(96 h LC 50) groups were similar,according to the"up-down-up"cyclical fluctuation law,the 1/4 φ(96 h LC 50) group showed bimodal trends on the fourth day and the eighth day.The MDA content of the 1/ 256 φ(96 h LC 50) group showed a single peak trend on the sixth day,the trends of the 1/16 and 1/4 φ(96 h LC 50) groups showed"up-flat-up",on the early(2 d) and late(10 d) stage of exposure,the MDA content of the groups of all concentrations showed an effect-dosage relationship,the greater the exposure concentration,the stronger the oxidative damage to the body.The exposure experiment results suggested that CAT activity fluctuated markedly,it indicated the the body suffered from a strong oxidative damage,different levels of oxidative stress appeared in the liver tissue of Cyprinus carpio.When the wastewater concentration reached a certain level,the body oxidative damage was obvious.When the oxyteracycline concentration exceeded the load range of free radical scavenging,the significant oxidative damage would appear again.CAT activity and MDA content were more sensitive to mixed wastewater effluent of penicillin and oxytetracycline,it will be able to reflect the oxidation stress,so it is suitable for aquatic biological index of monitoring penicillin and oxytetracycline pollution in water environment.
出处
《江西农业大学学报》
CAS
CSCD
北大核心
2013年第3期587-592,共6页
Acta Agriculturae Universitatis Jiangxiensis
基金
国家环保部公益课题项目(201109004)
河北省科技厅课题项目(12276708D)
河北省教育厅重点课题项目(ZH2011119)
河北省重点学科建设基金项目
关键词
抗生素
混合废水
出水
锦鲤鱼
抗氧化酶
丙二醛
antibiotics
mixed wastewater
effluent
Cypinus carpio
antioxidant enzymes
malondialdehyde(MDA)