摘要
目的:探讨桔梗总皂苷对人肺腺癌A549细胞周期及增殖抑制作用的影响。方法:体外培养肺腺癌细胞系A549细胞,MTT法检测桔梗总皂苷对人肺腺癌A549细胞增殖的影响;Giemsa染色法观察细胞形态学变化;流式细胞术检测桔梗总皂苷对A549细胞周期的影响。结果:MTT法检测显示,桔梗总皂苷能明显抑制人肺腺癌A549细胞的增殖,其抑制作用随药物浓度的增加而增强,呈剂量依赖关系(P<0.05)。流式细胞仪分析检测提示不同浓度的桔梗总皂苷均能促细胞凋亡,细胞周期被阻滞于G0/G1期,其凋亡率随药物浓度增高而增加(P<0.05)。结论:桔梗总皂苷能抑制人肺腺癌A549细胞增殖。
Objective： To investigate the inhibiting effects of total platycodin on cell cycle and proliferation of human pulmonary adenocarcinoma A549 ceils. Methods： The pulmonary adenocarcinoma A549 cells were cultred in vitro. The proliferation, variation of cytomorphology and total platycodin were detected by MTT, Giemsa staining and FCM （flow cytometry） methods respectively. Results： The results showed that cell proliferation was inhibited apparently by total platycodin with dose-effect relationship （P〈0.05）. The cytomorphology of A549 cells treated by total platycodin became shrinkage, turned round, floated, arranged loosely and dissolved under microscope. The FCM results showed that various concentrations of total platycodin could promote cell apoptosis and cell cycle could be blocked in G0/G1 period with dose-effect relationship （P〈0.05）. Conclusion： The human pulmonary adenocarcinoma A549 cell could be inhibited by total platycodin with dose-effect relationship. The mechanism of inhibiting effects should be further studied in vivo.
出处
《中华中医药杂志》
CAS
CSCD
北大核心
2013年第7期2135-2137,共3页
China Journal of Traditional Chinese Medicine and Pharmacy
基金
国家自然科学基金(No.30801496)
湖南省教育厅科研基金(No.09C722)~~
