摘要
目的探讨分区式脊髓导管联合骨髓基质干细胞在大鼠脊髓横断损伤模型中的修复作用。方法分区式脊髓导管联合骨髓基质干细胞桥接大鼠T8脊髓5mm全横断损伤，术后3个月观察修复效果。结果电生理检测显示皮层运动诱发电位的潜伏期（5．50±0．35）ms，与对照组比较较短，振幅（65．9±10．2）IxV，比对照组大（P〈0．05）；对桥接中间段进行神经微丝蛋白（NF）免疫荧光组织化学染色，可见再生神经纤维有序排列，电镜观察可见新形成的有髓神经纤维数量为11±4，高于对照组（P〈0．05）。结论分区式脊髓导管联合骨髓基质干细胞可桥接脊髓横断造成的缺损，促进有髓神经纤维再生，重建下行运动神经通路，部分恢复电生理特性。
Objective To study the effect of the multi-regional spinal cord conduit combine with bone marrow stromal cells （BMSCs） on repairing 5mm transection injury in rat spinal cord. Methods The multi-regional spinal cord conduit was combined with BMSCs and transplanted for connecting T8 spinal cord transection injury in rats. The repairing effect was evaluated 3M after surgery. Results Electrophysi- ology tests showed that the latency periods of the motor evoked potentials in cortex was （5.50 ± 0. 35 ） ms, shorter than that of the control groups, and the amplitude was （65.9 ± 10. 2） p^V, higher than that of the control groups （ P 〈 0. 05 ）. Neurofilament protein （ NF ） fluorescence immunohistochemistry staining showed that there were regenerated nerve fibers extending orderly in the middle part of the bridge site. The electron microscopy observation showed the number of the newly formed myelinated nerve fibers was 11 ± 4 and more than that of the control groups （ P 〈 0. 05）. Conclusion The multi-regional spinal cord conduit combining with BMSCs could bridge the defect caused by the transection of the spinal cord and promote the myelinated nerve fibers regenerate. The descent motor nervous pathways were reconstructed and the electro- physiological characteristics were partly restored.
出处
《中华实验外科杂志》
CAS
CSCD
北大核心
2013年第7期1403-1405,共3页
Chinese Journal of Experimental Surgery
基金
基金项目：国家自然科学基金资助项目（30870643）
江苏省高校自然科学研究项目（11kjb310007）
无锡市医学科技发展基金资助项目（YGM1107）
江苏省高校优势学科建设工程资助项目
关键词
分区式脊髓导管
骨髓基质干细胞
脊髓损伤
大鼠
Multi-regional spinal cord conduit
Bone marrow stromal cells
Spinal cord injury
Rat
