摘要
目的探讨神经干细胞(NSCs)与嗅鞘细胞(OECs)移植对脊髓全横断大鼠后肢运动功能的影响.方法SD大鼠行T11脊髓全横断术后,随机分为NSCs移植组、OECs移植组、联合移植组和对照组.术后将取自绿色荧光蛋白(GFP)转基因胚胎小鼠海马和新生小鼠嗅球的、经培养鉴定后的NSCs和OECs,以明胶海绵做载体分别或联合移植到各移植组动物脊髓损伤处,对照组不移植细胞,用BBB评分法评价1～8周大鼠后肢运动功能恢复的程度.8周后移植各组动物行左心室主动脉内4%多聚甲醛灌注,取横断处上下各约1 mL脊髓,连续冰冻切片(片厚20μm),置于荧光显微镜下观察切片中有无绿色荧光细胞,确定NSCs及OECs的存活情况.结果NSCs及OECs细胞在移植脊髓损伤后能存活,并向周围迁移;术后3和4周末,NSCs组、OECs组和联合移植组大鼠运动功能均较对照组有明显改善(P<0.05);且3周时,联合移植组和OECs组比NSCs组后肢运动功能变化显著(P<0.05);而联合移植组和OECs组比较后肢运动功能未见显著差别(P>0.05).结论NSCs、OECs和联合移植在早期均可促进脊髓全横断大鼠运动功能恢复;OECs组、联合移植组效果均优于NSCs组;联合移植对大鼠运动功能恢复在早期没有显现最佳作用.
[Abstract] Objoctivo To explore the engrafted effect of NSCs combined with OECs on the motor function repair of l^indlimbs in rats subjected to spinal cord transected injury. Mothods The NSCs and OECs were isolated from the Transgenic Green Fluorescent Protein （GFP） embryo mouse＇ hippocamp and neonatal mouse＇ olfactory bulb.Rats were divided into NSCs group, OECs group and co-transplantation group as well as control group. The recovery of posterior limb motor function in rats subjected to spinal cord transected injurywas evaluated by the BBB locomotion score respectively in each weekend among eight weeks. This was followed by a histochemiscal observation to know the engrafted cells survival and migration in the spinal cord of host under the fluo^seenee microscope. FIosults The NSCs and OECs cells survived and migrated in the spinal cord of transplantation groups： the motor functional improvement of hindlimb in rats was seen in all cell transplanted groups; NSCs group, OECs group and co-transplantation group demonstrated a significant increase than that of control group at 3 and 4 weeks post operation （P 〈 0.05） ; The changes of BBB scores in OECs group and co-transplantation group were more obvious than seen in the NSCs group, especially at three weeks （P 〈 0.05） ; There was no obvious difference between the OECs group and co-transplantation group in observed time points （P 〉 0.05）. Conclusions The engrafted NSCs, OECs and co-transplantation can improve partly the motor function recovery of hindlimb in rats at 3 to 4 weekm following cord transection; The OECs group and co-transplantation have better effects than that of NSCs group, but there is no obvious difference between the OECs group and co-transplantation group at observed time point in this investigation.
出处
《昆明医学院学报》
2007年第6期19-23,共5页
Journal of Kunming Medical College
关键词
脊髓损伤
神经干细胞
嗅鞘细胞
大鼠
Spinal cord injury
Neural stem cells
Olfactory ensheathing cells
Rat
