摘要
目的观察胆固醇损伤人血管内皮细胞(human umbilical vein endothelial cell,HUVEC)后能否影响血管平滑肌(vascular smooth muscle,VSMC)的增殖与迁移。方法用12.5、25.0、50.0 mg/L 3个浓度的胆固醇作用体外培养的HUVEC 24 h后,再用培养内皮细胞的培养基继续培养VSMC 24 h,CCK-8、划痕实验检测VSMC增殖与迁移;用12.5、25.0、50.0 mg/L的胆固醇作用共培养的HUVEC、VSMC 24 h,用Transwell检测VSMC迁移。结果 25.0、50.0 mg/L胆固醇作用HUVEC后的培养基与对照组相比(1.37±0.01),明显促进VSMC增殖(1.53±0.06)、(1.54±0.10)(P<0.05);12.5、25.0、50.0 mg/L胆固醇作用HUVEC后的培养基与对照组相比(33.24±0.43)均能促进VSMC细胞迁移[(269.10±3.78)、(272.79±4.69)、(458.69±4.78),P<0.01]。胆固醇作用共培养的HUVEC、VSMC 24 h后,促进VSMC迁移[(175.00±4.63)、(182.00±4.13)、(207.00±5.59)],与对照组相比(101.00±2.33)差异明显(P<0.01)。结论胆固醇诱导HUVEC损伤后能够促进VSMC增殖与迁移。
Objective To observe the effect of human umbilical vein endothelial cell （HUVEC） injury induced by cholesterol on human aortic vascular endothelial cell （HA-VSMC） proliferation and migration. Methods HUVEC were treated with cholesterol at 12.5,25.0 and 50.0 mg/L for 24 h, and then the VSMCs were incubated with the medium of cultured HUVEC for 24 h. CCK-8 and scratch assay was applied to analyze VSMC viability and migration. HUVEC and VSMC was co-cultured and treated with cholesterol at 12.5, 25.0 and 50.0mg/L for 24 h. VSMC migration was detected by Transwell assay. Results Compared with the control group （1.37±0.01 ）, the proliferation of VSMCs incubated with the culture medium of HUVEC treated with cholesterol at 25.0 mg/L and 50.0 mg/L increased significantly （ 1.53 ± 0.06 and 1.54 ± 0.10, P 〈 0. 05）. The culture medium of HUVEC subjected to cholesterol at 12.5, 25.0 and 50.0 mg/L could induce VSMC migration （269.10 ±3.78,272.79 ±4.69 and 458.69 ±4.78）, which were significantly different from that of the control group （33.24 ± 0.43, P 〈 0.01 ）. The co-culture of HUVEC and VSMC treated by choles terol induced VSMC migration （ 175.00 ±4.63, 182.00±4.13 and 207.00 ±5.59） , which were significantly different from that of the control group （101.00 ±2.33, P〈0.01）. Conclusion HUVEC, which is injured by cholesterol, can induce VSMC proliferation and migration.
出处
《第三军医大学学报》
CAS
CSCD
北大核心
2013年第13期1362-1365,共4页
Journal of Third Military Medical University
基金
贵州省科技厅自然科学基金[黔科合J字(2009)2-178]~~
关键词
动脉粥样硬化
血管内皮细胞
血管平滑肌细胞
胆固醇
atherosclerosis
human umbilical vein endothelial cell
human aortic vascular endothelialcells
cholesterol
