摘要
目的:探讨miR-21与肾癌转移的相关性,及miR-21对肾癌Caki-1细胞侵袭能力的影响。方法:实时PCR检测原发未转移肾癌和原发伴转移肾癌组织中miR-21的表达。将miR-21的前体pre-miR-21和抑制物anti-miR-21分别转染肾癌Caki-1细胞,实时PCR验证转染效果,然后检测转染后细胞的侵袭能力。结果:与原发未转移肾癌相比,原发伴转移肾癌组织中miR-21的表达显著上调;pre-miR-21和anti-miR-21转染后能够显著升高和降低Caki-1细胞miR-21的表达量;pre-miR-21组穿透滤膜的细胞数明显增加,而anti-miR-21组穿透滤膜的细胞数明显减少。结论:miR-21与肾癌的侵袭转移相关,miR-21能够促进肾癌细胞侵袭,在肾癌中具有促进转移的作用。
Objective： The present study aims to investigate the relationship between miR-21 and renal cancer metastasis as well as the influence of miR-21 on the invasiveness ability of renal cancer Caki-1 cells. Methods： Real-time PCR was performed to detect miR-21 in primary renal cancer without and with metastasis. Pre-miR-21 and anti-miR-21 were transfected to Caki-1 cells, respectively, and real-time PCR was used to detect transfection effects. Finally, invasiveness changes in Caki-1 cells were detected after transfection. Results： miR-21 expression in primary renal cancer with metastasis was much higher than that in primary renal cancer without metasta- sis. After transfection, pre-miR-21 and anti-miR-21 significantly increased and decreased the miR-21 expression in Caki-1 cells, respec- tively. The transmembrane cells of the pre-miR-21 group increased significantly, whereas those of the anti-miR-21 group decreased significantly. Conclusion： miR-21 and renal cancer metastasis are related, miR-21 advances renal cancer cell invasion and metastasis.
出处
《中国肿瘤临床》
CAS
CSCD
北大核心
2013年第12期702-704,共3页
Chinese Journal of Clinical Oncology
基金
国家自然科学基金资助项目(编号:30901480
81172408)
辽宁省教育厅科学技术研究项目(编号:L2011130)资助~~
