摘要
目的:探讨凋亡相关性微小RNA(microRNA,miRNA)在心肺复苏(CPR)后大鼠心肌中的表达变化。方法:雄性SD大鼠16只,随机分成对照组(A组)和复苏组(B组),每组8只,采用窒息法建立心搏骤停(CA)大鼠模型。以脱氧核糖核苷酸末端转移酶介导的缺口末端标记(TUNEL)法检测自主循环恢复后(ROSC)后24 h的心肌细胞凋亡指数(AI);实时荧光定量法检测miRNA-1和miRNA-133a的表达情况;免疫组化染色法检测Bcl-2、Bax蛋白表达。结果:B组ROSC后24 h AI、miRNA-1、miRNA-133a、Bcl-2及Bax蛋白表达比A组高(P<0.01),Bcl-2/Bax值比A组低(P<0.01),miRNA-1、miRNA-133a分别和Bcl-2、Bax的变化有相关性。结论:大鼠心搏骤停/心肺复苏后存在明显的心肌细胞凋亡,凋亡相关的miRNA变化可能是其机制之一。
Objective： To explore changes of the expression of apoptosis-related MicroRNAs, target genes and protein in cardiomyocytes after cardiopulmonary resuscitation in rats. Methods： Sixteen male SpragueDawley mice were randomly divided into two groups： the control group （n=8） and CPR group （n=8）. The animal model of cardiac arrest （CA） induced by asphyxia was established and CPR was performed. Myocardium samples were taken 24 hours after CPR or intubation for detecting the expression profile of microRNA-1 and miRNA-133a using RT-PCR. Myocardium cell apoptosis were detected by TUNEL method. The levels of Bcl-2 and Bax were measured using immunohistochemical staining. Results： Compared with group A, microRNA-1, miRNA-133a, the levels of bcl-2 and Bax protein increased significantly in group B （P 〈 0.01）, and Bcl-2/Bax ratio decreased significantly （P 〈 0.01）. There was significant correlation between miRNA-1, miRNA-133a and Bcl-2, B ax individely. Conclusion： There are promoting cardiomyocyte apoptosis after CA/CPR. The changes of apoptosis-related microRNAs may be one of the mechanisms.
出处
《温州医学院学报》
CAS
2013年第6期367-370,共4页
Journal of Wenzhou Medical College
基金
温州市科技计划项目(Y20090241
Y20080084)
