摘要
应用高效液相色谱示差折光检测法测定β-淀粉酶酶解淀粉产物中葡萄糖、麦芽糖和麦芽三糖的含量,出峰分离效果显著,重现性较好。葡萄糖、麦芽糖和麦芽三糖的RSD分别为2.08%,2.41%,2.61%。葡萄糖、麦芽糖和麦芽三糖加标后回收率分别在101.3%,98.4%,93.5%,回收率较高。4种植物淀粉酶解产物中,甘薯淀粉酶解产物中葡萄糖含量最低。
Starch was hydrolyzed by heat-stable a-amylase, β-amyl- ase. Their hydrolysates were determined by HPLC with differential refractometer detector. The hydrolysates of starch consisted mainly of maltose and maltotriose, trace amount glucose, and no sucrose. In the HPLC method, a good separation of hydrolysates was a chieved. The determination of hydrolysates showed good reproduc- ibility, and the RSD values of glucose, maltose and maltotriose were 2. 08%, 2. 41% and 2. 61%, respectively. The recoveries of glu cose, maltose and maltotriose were 101.3%, 98.4% and 93.5%, respectively. Among the 4 kinds of starch hydrolysates, that from the sweet potato starch by β-amylase was the lowest.
出处
《食品与机械》
CSCD
北大核心
2013年第3期86-88,共3页
Food and Machinery
基金
国家自然科学基金项目(编号:311641/C200101)
河南省科技攻关计划项目(编号:122102110037)
河南科技学院食品生物技术重点实验室培育计划项目(编号:11008)
