摘要
目的:探讨PCR+膜杂交法CT/UU/NG三联检测在临床性病筛查中的应用价值。方法:分别采用PCR+膜杂交法和实时荧光PCR法检测CT、UU、NG,并分析比较两种方法的一致性以及相关性。结果:PCR反向膜杂交法和实时荧光PCR法的CT、UU、NG的筛查结果接近(P>0.05),而男女筛查结果差异较大(P<0.05)。结论:PCR反向膜杂交法操作简便,筛查快速、准确,有与实时荧光PCR法相当的检测准确性,具有较高的临床应用价值,值得在临床中推广应用。
Objectives： To study the clinical application value of PCR ＋ membrane hybridization method CT/UU/NG de- tection in STD screening. Methods： We use PCR ＋ membrane hybridization method to detect CT, UU and NG. We also use real -time fluorescent quantitative PCR to detect CT, UU and NG. We compare the results of the two approaches. Results： The results of both methods showed no significant difference （ P 〉 0. 05 ）. Men＇ s screening results were significantly different from those of women （ P 〈 0. 05 ）. Conclusion： PCR membrane hybridization is a simple and rapid method which is as accurate as real - time fluorescent PCR. It is of high value for clinical application.
出处
《中国性科学》
2013年第6期38-40,共3页
Chinese Journal of Human Sexuality
