摘要
【目的】探索商品化β-葡聚糖酶水解小麦秸秆制备纤维寡糖的最佳条件,为纤维寡糖的工业化生产提供科学依据。【方法】采用浓硫酸和浓盐酸混酸降解微晶纤维素制备纤维寡糖混合物,通过分离纯化得到聚合度为2—5的纤维寡糖单一组分。用所得纤维二糖作为标准品测定小麦秸秆酶解产物中的纤维二糖,并以纤维二糖含量和小麦秸秆转化率为衡量指标,研究酶解反应温度、pH、反应酶底比E/S、反应底物浓度、反应时间对寡糖生成的影响。【结果】采用活性炭柱层析法得到了高纯度的聚合度2-5的纤维寡糖单一组分;当酶解反应温度为50℃、pH为5.5、E/S为0.4、底物浓度为2%、时间10 h时酶解效果最好,小麦秸秆总转化率达到55.57%,纤维二糖得率为148.15 mg.g-1秸秆。【结论】活性炭柱层析可以很好地分离纤维寡糖,得到了聚合度为2—5的纤维寡糖单一组分;酶解小麦秸秆总转化率及纤维二糖得率均提高。
[ Objective ] The aim of this study was to explore the optimum conditions of wheat straw degradation by β-glucanase. [Method] The mixed-acid hydrolysis and chromatography were selected for the separation process, in which the cello- oligosaccharides with degrees of polymerization from 2 to 5 were obtained. The single factor experiments for optimal conditions were temperature, pH, E/S concentration of wheat straw and the hydrolysis time. The optimal conditions for hydrolysis of wheat straw were obtained through analyzing the yield of cellobiose and the conversion rate of wheat straw. [Result] The experimental results showed that by activated carbon column chromatography, the standard cello-oligosaccharides were obtained and the optimum conditions for wheat straw hydrolysis were as follows： the reaction temperature was 50℃, pH was 5.5, E/S was 0.4, concentration of wheat straw was 2%, and hydrolysis time was 10 h. Using the optimum conditions, the cellobiose yield was 148.15 mg.g-1 wheat straw and the conversion rate of wheat straw was 55.57%. [Conclusion] The yield ofcellobiose and the conversion rate of wheat straw were both increased under the optimum conditions. It was found that the chromatographic columns composed of charcoal could separate well cello-oligosaccharides with degrees of polymerization from 2 to 5.
出处
《中国农业科学》
CAS
CSCD
北大核心
2013年第11期2345-2352,共8页
Scientia Agricultura Sinica
基金
农业部"948"重点项目(2011-G7)
科技部国家科研院所科技开发专项(2010EG134290)
关键词
小麦秸秆
纤维二糖
总转化率
酶解
wheat straw
cellobiose
percent conversion
hydrolysis
