摘要
目的:研究磷酸锌矿化胶原膜对人成骨样细胞MG63增殖及分化的影响。方法:制备磷酸锌矿化胶原膜并在其上接种MG63成骨细胞株,扫描电镜观察MG63细胞在胶原膜表面的生长情况,采用CCK-8及碱性磷酸酶(ALP)试剂盒检测MG63细胞的增殖及分化能力。结果:扫描电镜下可见胶原膜表面有大量晶体样沉积物,在其上培养1 d后MG63细胞粘附于胶原膜表面,开始向各方向伸出伪足,与胶原膜相互交联。与阴性对照组相比,磷酸锌矿化胶原膜于第3天明显促进MG63细胞增殖,于第1、5、7 d无显著影响;于第1 d可提高碱性磷酸酶活性,促进MG63细胞分化,于第7、14 d无显著影响。结论:磷酸锌矿化胶原膜具有良好的生物相容性,可促进MG63成骨细胞的早期增殖及分化。
Objective:This study was to determine the effect of zinc phosphate mineralized collagen membranes on the proliferation and differentiation of human osteoblast-like cells MG63 in vitro.Method:The collagen membranes were mixed with zinc phosphate by the method of immersing into the mineralization solution.Cells from an established osteoblast-like line(MG63)were cultured on membranes.Cell morphology attached to the membranes was evaluated by scanning electron microscope.The quantity and ALP activity of cells were determined by CCK-8 assay and ALP activity test.Result:MG63 cells attached to the membranes and extended pseudopod.Compared to the control,zinc phosphate mineralized collagen membranes significantly increased the proliferation of MG63 on the 3rd day and no significant influence on the other cultured days was observed.The ALP activity of MG63 planted on zinc phosphate mineralized collagen membranes was significantly higher than the control on the 1st day,while no significant influence on the other cultured days was observed.Conclusion: Zinc phosphate mineralized collagen membranes possess excellent biocompatibility.
出处
《临床口腔医学杂志》
2013年第6期323-326,共4页
Journal of Clinical Stomatology
基金
国家自然科学基金面上项目(30973355)
广东省自然科学基金(S2011010004978)
关键词
磷酸锌
胶原膜
MG63成骨细胞
增殖
碱性磷酸酶
Zinc phosphate
Collagen membranes
MG63 osteoblast
Proliferation
Alkaline phospatase
