摘要
目的探讨磷酸化MAPK[磷酸化细胞外信号调节蛋白激酶1／2（p-ERKl／2）、磷酸化蛋白激酶p38（p-p38）、磷酸化c．Jun氨堆末端激酶（p-JNK）]表达在光气吸人所致肺损伤中的作用及其与基质金属蛋白酶9（MMP一9）的关系。方法选取30只雄性Wistar大鼠．按随机数字表法分为空气对照组（简称C组）、光气吸入组（简称P组）、PD98059（ERKl／2特异性阻断剂）组、SB203580（p38特异性阻断剂）组和SP600125（JNK特异性阻断剂）组，每组6只。计数支气管肺泡灌洗液（BALF）中性粒细胞，测定肺湿干比，ELISA法检测血清炎症因子TNF—d、IL-1B、IL-6、IL-8的表达水平．蛋白质印迹法检测各组肺组织中p-ERKI／2、p-p38、p-JNK、MMP-9的蛋白表达，实时荧光定量PCR法检测各组肺组织中MMP-9mRNA表达。组问整体比较采用单因素方差分析，两两比较采用SNK法。结果与C组[中性粒细胞数为（2．0±0．7）×10^4个／ml，肺湿干比为3．7±0．6]相比，P组BAI。F中性粒细胞数[（10．7±1．4）×10^4个／mL]增多，肺湿干比（7．6±0．4）升高；而SB203580组、SP600125组BAI．F中中性粒细胞数分别为（8．3±1．1）X10^4、（7．9±1．3）×10^4个／mL，肺湿于比各为6．1±1．4、6．1±0．9，较P组均显著降低（P值均小于0．01）。P组炎症因子TNF-a、IL-1B、IL-6、IL-β的表达较C组升高，而SP600125组和SB203580组的表达水平较P组有不同程度降低，但仍高于c组，差异均有统计学意义（P〈0．05或P〈0．01）。，蛋白质EI]迹法结果显示，P组p-p38、p-JNK的表达最分别是1．19±0．22、1．43±0．14，较C组的0．76±0．06、0．74±0．05明显增强；P1）98059组、SB203580组、SP600125组P—ERKl／2、p-p38、p-JNK蛋白表达量各为0．47±0．05、0．88±0．07、0．91±0．07，与P组比较均明显降低（P〈0．05或P〈0．01）。P组MMP-9蛋白表达量（2．23±0．I8）及mRNA表达量（4．93±0�
Objective To investigate the effects of phosphorylated mitogen-activated protein kinases（MAPK） , including the phosphorylated extracellular signal-regulated protein kinase 1/2 （p-ERK1/2） , the phosphorylated protein p38 （p-p38） , the phosphorylated c-Jun N-terminal kinase （p-JNK） , on phosgene inhalation-induced lung injury and its relationship with matrix metalloproteinase 9 （MMP-9）. Methods According to the random number table, 30 male Wistar rats were divided into air control group （C） , phos- gene inhalation group （P） , PD98059 （ specific inhibitor of ERK1/2） group, SB203580 （ specific inhibitor of p38） group, and SP600125 （specific inhibitor of JNK） group, with 6 rats in each group. The number of neutrophils in the bronchoalveolar lavage fluid （BALF） was counted and the lung wet-dry ratio （W/D） was examined. The serum levels of inflammatory factors TNF-a, IL-1β, IL-6, and IL-8 were determined with ELISA. The protein expressions of p-ERK1/2, p-p38, p-JNK, and MMP-9 in lung tissue were detected with Western blotting. The mRNA level of MMP-9 in lung tissue was detected with real-time fluorescence quantitative PCR. Data were processed with one-way analysis of variance （among groups） and SNK method （paired comparison）. Results Compared with those of group C [ respectively （2.0 ± 0.7 ） x 104/mL and 3.7 ± 0.6] , the number of neutrophils and W/D of group P [ respectively （ 10.7 ± 1.4） 10^4/mL and 7.6 ±0. 4 ] were increased. The number of neutrophils in group SB203580 and group SP600125 was respectively （8.3 ± 1.1） 10^4 , （7.9 ± 1.3） 10^4/mL, with W/D respectively 6.1 ±1.4, 6.1 ±0.9, all of which decreased as compared with those of group P （with P values all below 0.01 ）. Compared with those of group C, the levels of TNF-a, IL-113, IL-6, and IL-8 of group P were increased, but decreased in group SB203580 and group SP600125 compared with that of group P, though still higher than those of group C, and the differ- ences were statist
出处
《中华烧伤杂志》
CAS
CSCD
北大核心
2013年第3期261-266,共6页
Chinese Journal of Burns
基金
复旦大学青年科学基金（09FQ91）
复旦大学生物医学院IBS平台资助项目
关键词
烧伤
化学
烧伤
吸入性
光气
肺损伤
基质金属蛋白酶9
丝裂原活化蛋白激酶
Burns, chemical
Burns, inhalation
Phosgene
Lung injury
Matrix metallo-proteinase 9
Mitogen-activated protein kinase
