摘要
目的观察乌司他丁注射液对光气引起急性肺损伤时肿瘤坏死因子-α（TNF-a）表达的影响。方法清洁级健康SD雄性大鼠64只，以随机数字表达法分为两大组共8个小组，即染毒组（乌司他丁干预组、光气染毒组、生理盐水对照组）和未染毒组（空气对照组、生理盐水空气对照组、乌司他丁空气对照组），每组各8只。空气对照组、生理盐水空气对照组、乌司他丁空气对照组吸入空气，光气染毒组、乌司他丁干预组和生理盐水对照组吸入同等浓度和相同时间的光气，并分别同等容积、不同种类和不同浓度的药物从尾静脉注射到大鼠体内并测量肿瘤坏死因子的变化情况，酶联免疫法测定肿瘤坏死因子的含量变化，荧光定量PCR测定TNF-amRNA的表达情况。结果实验大鼠64只全部进入结果分析：损伤组右下肺湿质量、干质量及湿／干质量比较空气对照组和乌司他丁空气对照组显著增加（P〈0．01）；乌司他丁治疗组和地塞米松治疗组右下肺湿质量、干质量及湿／干比值较模型组明显降低（P〈0．01）但仍明显高于对照组（P〈0．01）；对照组的肺组织大体观察可见表面光滑呈淡红色无充血水肿及梗死灶；光气染毒组和生理盐水组可见肺微血管出血和微血栓形成，肺问质和肺泡内有水肿液和炎症细胞的浸润，部分可见灶状肺不张，乌司他丁治疗组和地塞米松治疗组的病理学改变可见肺微血管充血出血微血栓形成及炎症细胞浸润，但比光气染毒组要稍轻；乌司他丁治疗组肿瘤坏死因子α的表达水平均高于空气对照组和乌司他丁空气对照组（P〈0．01），但低于光气染毒组和生理盐水组（P〈0．01）。结论乌司他丁可能通过抑制TNF-α的高表达来促进急性肺损伤的修复。
Objective To observe the effects of ulinastatin on the expressions of tumor necrosis factor-α（TNF-α） in the lung tissues of rats with acute lung injury induced by phosgene, and to explore the mechanism of ulinastatin in treating acute lung injury. Method Sixty-four clean grade healthy male SD rats were randomly divided （random number） into eight groups with eight in each group. Group A1 in which rats were exposed to air. Group A2 in which rats wereexposed to air and treated with saline. Group A3 in which rats were exposed to air and treated with dexamethasone. Group A4 in which rats were exposed to air and treated with ulinastatin. Group B1 in which rats were exposed to phosgene without treatment. Group B2 in which rats were exposed to phosgene and treated with saline. Group B3 in which rats were exposed to phosgene and treated with dexanlethasone. Group B4 in which rats were exposed to phosgene and treated with ulinastatin. The expressions of TNF-α in the lung tissues were measured by using immunohistocheistry. Lung tissues were observed grossly and under 200-fold light microscope to identify the positive expressions in kytoplasm. Results In group B1 and group B2, the wet weight, dry weight and wet/dry weight ratio og right lower lobe of lung were higher than those in group A1 and group A4 （ P 〈 0.01 ）, and those in group B4 and group B3 were significantly lower than those in group B1 （ P 〈 0.01 ）, but still higher than those in group A1 and A4 （ P 〈 0.01 ）. The gross observation suggested that the surfaces of lung tissues in group Aland group A2 were slick and rose pink without congestion, dropsy or infarction; the surfaces of lung tissue in groups B1 and B2 appeared with congestion, dropsy and many petechia. The surfaces of lung tissue in groups B3 and B4 were similar to those in groups B1 and B2. Under the light microscope, the structure of lungs in groups AI and A2 were clear without congestion, effusion or inflammatory cell infiltration. In groups B1 and B2, the engorgement of l
出处
《中华急诊医学杂志》
CAS
CSCD
北大核心
2010年第1期37-42,共6页
Chinese Journal of Emergency Medicine
基金
江苏省教育厅开放课题资助项目（KJS050004）
上海市科委科研资助项目（54119610）
上海市公共卫生重点学科资助项目（08gwzx0404）
