摘要
该文鉴定了一种提取物具有维持和增强细胞存活与分化的能力,这将在细胞生物学研究中得到应用。制备四种卵细胞提取物,雄鼠的脾细胞被提取物渗透并培养,然后输注给雌鼠。在移植后雌鼠外周血中GFP阳性细胞和骨髓细胞中的Y染色体被检测,对比脾细胞在体内存活与分化的不同能力。移植蛋白和全蛋提取液处理的脾细胞的雌鼠外周血检测到GFP阳性细胞,在移植后120 d,雌鼠骨髓中检测到的Y染色体阳性率与外周血中的GFP阳性细胞率呈正比。用蛋白和全蛋提取液渗透脾细胞后,脾细胞在雌鼠体内存活能力强于其他提取液。结果表明,鸡蛋白提取液有维持并增强脾细胞存活的作用。因此蛋白提取物可在将来用于干细胞功能的维持。
This paper concerns the identification of an extract with the ability to maintain and enhance the survival and differentiation of cells which will be useful in cellular biology research. Four types of egg extracts were prepared. The spleen cells from male mice were permeabilized with the extracts and cultured respectively, and then transfused into female mice. The GFP-positive cells in the peripheral blood of female mice and Y chromosome in bone marrow cells were detected to compare the different abilities of spleen cells to survive and differentiate in vivo. Surviving GFP-positive spleen cells were detected in the female mice of chicken-egg-white and whole-egg extracts group. At 120 days after transplantation, the percentage of cells containing a Y chromosome in the bone marrow positively correlated with the percentage of GFP-positive cells in the peripheral blood. After permeabiliza- tion by chicken-egg-white or whole-egg extracts, spleen cells demonstrate significantly enhanced survivability. The results show the vital role of chicken-egg-white extracts in maintaining and enhancing the survival of spleen cells. Therefore, the chicken-egg-white extracts may be of use in future stem cells study.
出处
《中国细胞生物学学报》
CAS
CSCD
北大核心
2013年第6期804-809,共6页
Chinese Journal of Cell Biology
基金
国家自然科学基金(批准号:31172170)
国家重点基础研究发展计划(973计划)(批准号:2012CB518106)
中国博士后科学基金(批准号:201104748)资助的课题~~
关键词
GFP转基因鼠
脾细胞
提取物
细胞存活
细胞分化
GFP-transgenic mice
spleen cells
extracts
cell survival
cell differentiation
