摘要
目的:建立测定健胃消炎颗粒中大黄素和大黄酚含量的方法。方法:采用高效液相色谱法。色谱柱为大连依利特Sino-Chrom C18柱,流动相为甲醇-0.1%磷酸溶液(80:20,V/V),检测波长为254nm,柱温为30℃,流速为1.0ml/min,进样量为20μl。结果:大黄素、大黄酚检测质量浓度分别在1.1～5.5、2.85～14.25μg/ml范围内与峰面积积分值呈良好的线性关系(r=0.9997和0.9996);精密度、稳定性、重复性试验的RSD均≤1.76%;平均加样回收率分别为104.43%、108.04%,RSD分别为1.76%、0.62%(n=6)。结论:该法简单、准确、重复性好、专属性强,可用于健胃消炎颗粒的质量控制。
OBJECTIVE： To establish a method for the content determination of emodin and chrysophanol in Jianwei xiaoyan granules. METHODS： HPLC method was adopted. A SinoChrom C18 column of Dalian Elite was used with mobile phase consisted of methanol-0.1% phosphoric acid （80：20, V/V） with flow rate of 1.0 ml/min .The detection wavelength was set at 254 nm and col- umn temperature was 30 ℃. The sample size was 20 pl. RESULTS： The linear ranges were 1.1-5.5 μg/ml for emodin（r=0.999 7） and 2.85-14.25 μg/ml for chrysophanol （r=0.999 6）. RSDs of precision, stability and reproducibility tests were lower than and equal to 1.76%. Average recoveries were 104.43% （RSD=l.76%, n=6） and 108.04% （RSD=0.62%, n=6）, respectively. CONCLUSIONS： The method is simple, accurate, reproducible and specific, can be used for quality control of Jianwei xiaoyan granules.
出处
《中国药房》
CAS
CSCD
2013年第24期2285-2287,共3页
China Pharmacy
