摘要
目的建立小败毒膏的质量标准。方法采用薄层色谱法对小败毒膏中的大黄、黄柏、金银花、蒲公英、赤芍和陈皮进行定性鉴别;采用高效液相色谱法测定该制剂中大黄酸、大黄素、大黄酚和大黄素甲醚的含量,色谱柱为CAPCELLPAKC18(250mm×4.6mm,5μm)分析柱;流动相:甲醇-0.1%磷酸溶液(70∶30);流速:1.0mL/min;检测波长:254nm;柱温:30℃。结果薄层色谱法可鉴别出该制剂中的大黄、黄柏、金银花、蒲公英、陈皮和赤芍。大黄酸、大黄素、大黄酚和大黄素甲醚分别在0.0764～0.7640、0.0708～0.7080、0.0738～0.7380、0.0414～0.4140μg范围内有良好的线性关系,r分别为0.9998、1.0000、0.9997、0.9999,平均回收率分别为100.87%(RSD为1.61%),100.70%(RSD为0.93%),99.82%(RSD为1.62%),99.91%(RSD为1.60%),n=6。结论该方法准确、灵敏、重复性好,能有效地控制小败毒膏的质量。
Aim To establish the quality standard for Xiaobaidu Gao. Methods Radix Et Rhizoma Rhei, Cortex Phellodendri Chinensis, Flos Lonicerae Japonicae, Herba Taraxaci, Radix Paeoniae Rubra, Pericarpium Citri Reticulatae were identified by TLC. The contents of Rhein, Emodin, Chrysophanol and Rheochrysidn were determined by HPLC. SHISEIDO CAPCELL PAK C18 （250mm × 4.6mm,5μm） was used as the chromatographic column and methanol-0.1% phosphoric acid solution （70： 30） as the mobile phase. The detection wavelength was 254nm; the flow rate was 1.0mL/min, and the column temperature was 30℃. Results Radix Et Rhizoma Rhei, Cortex Phellodendri Chinensis, Flos Lonicerae Japonicae, Herba Taraxaci, Radix Paeoniae Rubra, Pericarpium Citri Reticulatae could be identified by TLC. Rhein, Emodin, Chrysophanol and Rheochrysidn showed a good linear relationship in a range of 0.076 4-0.764 0, 0.070 8 -0. 708 0,0. 073 8 -0. 738 0 and 0. 041 4 -0. 414 0μg, r =0. 999 8, 1.000 0,0. 999 7 and 0. 999 9,The average recovery was 100.87% （ RSD = 1.61% ）, 100.70% （ RSD = 0.93% ）,99.82% （ RSD = 1.62% ）,99.91% （ RSD = 1.60% ）, n =6. Conclusion The method is simple with good reproducibility and can be used as the control of Xiaobaidu Gao.
出处
《解放军药学学报》
CAS
2009年第1期52-55,共4页
Pharmaceutical Journal of Chinese People's Liberation Army
基金
国家药典委员会标准提高行动
