摘要
目的探讨切除修复交叉互补基因1(ERCC1)在胃癌的癌组织和外周血淋巴细胞(PBLC)中表达的相关性及其与临床病理特征的关系。方法采用免疫组化法检测53例胃癌术后癌组织和PBLC中ERCC1的表达,另取20例胃肠道良性疾病和正常人的PBLC作为对照。结果胃癌的癌组织、PBLC和对照组的PBLC中ERCC1的阳性表达率分别为67.9%、56.6%和10.0%,胃癌的PBLC中ERCC1的阳性表达率显著高于对照组(P<0.05)。在胃癌的癌组织和PBLC中,ERCC1表达与年龄、性别、病理类型、临床分期、T分期、淋巴结转移和分化程度均无关。胃癌的癌组织与PBLC中ERCC1的表达呈正相关(r=0.475,P=0.000)。结论通过检测胃癌的PBLC中ERCC1表达可以间接反映癌组织中ERCC1的表达状况。
Objective To investigate the correlation between excision repair cross-complementationl （ ERCC1 ） expression in tumor tissue and peripheral blood [ymphocytes （PBLCs） from gastric cancer, and their expression with clinical features. Methods A total of 53 patients with gastric cancer receiving surgery were studied. ERCC1 protein expression in tumor tissue and PBLCs were deter- mined by immunohistochemical staining. Another 20 cases of PBLCs from gastrointestinal benign disease and the health were collected as control. Results The positive expression rates of ERCC1 were 67.9% , 56. 6% and 10. 0% in tumor tissues, PBLCs of gastric cancer, and PBLCs of control group. The expression of ERCC1 in PBLCs of gastric cancer was much higher than that in PBLCs of con- trol group（ P 〈 0.05 ）. There was no relation between the ERCC1 expression and the clinical characteristics including age, gender, his- tological type, clinical stage, T stage, metastasis of lymph node and degree of differentiation. The PBLCs ERCC1 expression correlates with the expression in tumor tissues（r = 0. 475, P = 0. 000）. Conclusion In gastric cancer, the ERCC1 expression in PBLCs can in- directly reflect the ERCC1 expression in cancer tissues.
出处
《临床肿瘤学杂志》
CAS
2013年第5期398-402,共5页
Chinese Clinical Oncology
关键词
胃癌
切除修复交叉互补基因1
DNA修复
外周血淋巴细胞
免疫组织化学
Gastric cancer
Excision repair cross-complementationl （ERCC1）
DNA repair
Peripheral blood lympho-cyte
Immunohistochemistry
