摘要
目的:探讨右丙亚胺(DEX)对表柔比星(EPI)诱导乳腺癌细胞凋亡的影响。方法:分别以不同浓度EPI作用于MCF-7、MDA-MB-231和BT474 3种乳腺癌细胞系24h,用MTT法检测抑制率,选择EPI最佳实验浓度;应用1.0μg/mL EPI与1.0、10和20μg/mL DEX分别及联合作用于3种乳腺癌细胞系,用MTT法检测抑制率,用流式细胞术检测细胞凋亡。结果:1.0μg/mL EPI组3种乳腺癌细胞的抑制率分别为(48.72±0.34)%、(64.40±2.63)%和(58.57±2.23)%,1.0μg/mL EPI联合1.0μg/mL DEX组3种细胞的抑制率分别为(46.44±1.35)%、(65.20±0.21)%和(57.52±2.31)%,1.0μg/mL EPI联合10μg/mL DEX组3种细胞抑制率分别为(47.57±2.20)%、(61.70±1.08)%和(59.54±1.26)%,1.0μg/mL EPI联合20μg/mL DEX组3种细胞抑制率分别为(43.85±1.19)%、(60.80±0.62)%和(58.25±3.50)%,EPI和DEX两药联合组(EPI∶DEX=1∶1和1∶10)与EPI组3种乳腺癌细胞系的抑制率比较差异无统计学意义,P>0.05;两者浓度为1∶20时对3种乳腺癌细胞系的增殖影响差异有统计学意义,P<0.05。流式细胞术检测1.0μg/mL EPI组3种乳腺癌细胞凋亡率分别为8.54%、11.25%和10.78%,EPI∶DEX=1∶10组3种乳腺癌细胞凋亡率分别为8.74%、11.85%和10.49%,两组细胞凋亡率差异无统计学意义,P>0.05。结论:心脏保护剂量的DEX并不影响EPI诱导的乳腺癌细胞凋亡效果。
OBJECTIVE： To investigate the influence of epirubicin（EPI） cytotoxicity by dexrazoxane（DEX） in hu- man breast cancer cell lines. METHODS： EPI of different concentrations was added into the culture fluid of human breast cancer cells. MTT method was used to detect the inhibition rate. Three breast cancer cell lines were treated with EPI （1.0 μg/mL）,DEX（1.0,10 and 20 μg/mL）,or with EPI： DEX （1 ： 1,1 ： 10,1 ： 20）. MTTmethod was used to detect the inhibition rate. Flow cytometry was used to detect the apoptosis of the cells. RESULTS： The inhibition rates of group A（ 1.0μg/mL EPI） were （48.72± 0.34）~, （64.40±2.63）% and （58.57±2.23）% respectively. The inhibition rates of group E （1.0μg/mL EPI＋1. 0 μg/mL DEX） were （46.44± 1.35） %0, （65.20±0.21） % and （57. 522±2.31） % respec tively. The inhibition rates of group F （1.0μg/mL EPI＋10 μg/mL DEX） were （47.57±2.20） %, （61.70±1.08） % and （59.54±1.26） % respectively. The inhibition rates of group G （1.0 μg/mL EPI＋ 20 μg/mL DEX） were （43.85 ± 1.19）%,（60.80±0.62）%and （58.25±3.50）% respectively. The apoptosis rates of group e（1.0 μg/mL EPI） were 8.54±11.25% and 10.78%,while the apoptosis rates of group d（1.0 μg/mL EPI＋10 μg/mL DEX） were 8. 74%, 11.85% and 10.49%. Compared with the inhibition rate of 1.0 μg/mL EPI,the inhibition rates of 1.0 /zg/mL EPI corn bined with DEX（1.0,10 μg/mL） showed no statistical difference （P〉0.05）. While the 1.0 μg/mL EPI combined with20μg/mlJ DEX had significant influence on the proliferation of breast cancer cells （P〈0.05）. CONCLUSION： DEX given at cardioprotective doses does not alter the cytotoxicity efficacy of the EPI.
出处
《中华肿瘤防治杂志》
CAS
北大核心
2013年第12期914-917,共4页
Chinese Journal of Cancer Prevention and Treatment
