摘要
【目的】探讨血管内皮抑素（Endostatin，ES）对三阴性乳腺癌（TNBC）化疗增敏的作用。【方法】37℃务件下，体外培养TNBC的MDA—M13-231细胞，用不同浓度多烯紫杉醇单药和重组人ES（rhES）两药混合分别孵育TNBC细胞24h、48h，用MTT法检测细胞生长抑制率。【结果】多烯紫杉醇单药不同浓度（0．1μg／mL、1μg／mL、10μg／mL、100μg／mL和1000μg／mL、）对MDA—MB-231细胞24h半数抑制浓度（IC50）分别为：-8．6、-9．4、-6．36、1．6、-7。联合使用rh-ES（0．05μg／mL恩度）和多烯紫杉醇不同浓度（0．1μg／mL、1μg／mL、10μg／mL、100μg／mL，1000μg／mL多西紫杉醇）对MDA—MB-231细胞24hIC50分别为：1．23、1．29、1．26、-0．22和0．01。【结论】提高浓度并不能增加多烯紫杉醇对MDA-MB-231细胞的抑制率。rh—ES可以增加低浓度多烯紫杉醇对TNBC的MDA—MB-231细胞的敏感性。
[Objective] To explore the effect of endostatin（ES） on chemotherapeutic sensitization of triplenegative breast cancer（TNBC）. [Methods] TNBC MDA-MB-231 cells were cultured at 37℃, and incubated with different concentration of only docetaxel or mixed with recombinant human ES for 24h and 48h. MTT assay was used to detect the inhibition rate of cell growth. [Results] The inhibition rate of different concentration of docetaxel（0.1μg/mL, 1μg/mL, 10μg/mL, 100μg/mL and 1000μg/mL） on MDA-MB-231 cells were -8.6, -9.4, -6.36, 1.6 and -7, respectively. The inhibition rate of ES（0.05μg/mL） combined with different concentration of docetaxel（0.1μg/mL, μ1g/mL, 10μg/mL, 100μg/mL and 1000μg/mL） on MDA-MB- 231 cells were 1.23, 1.29, 1.26, -0.22 and 0.01, respectively. [Conclusion] Elevating the concentration can not increase the inhibition rate of docetaxel on MDA-MB-231 cells. Endostatin can increase the sensitivity of low concentration of docetaxel to TNBC MDA-MB-231 cells.
出处
《医学临床研究》
CAS
2013年第12期2307-2309,2312,共4页
Journal of Clinical Research
基金
辽宁省教育厅基金课题（编号：2009A722）
