摘要
目的观察丹参三七混合液口服对光动力学诱导大鼠实验性视网膜分支静脉阻塞的预防作用,并探讨作用机制。方法 20只BN大鼠分为实验组(丹参三七混合液灌胃)和对照组(生理盐水灌胃),每组10只。实验组按三七0.25 g/kg、丹参0.04 g/kg药量灌胃给药,对照组灌服等体积生理盐水,给药1个月后采血检测大鼠血小板黏附率和血小板聚集率,并采用光动力学方法诱导大鼠视网膜静脉阻塞模型,按10 mg/kg剂量尾静脉注射孟加拉红,1 min后,氙激光(参数:功率100 mW,直径50μm,曝光时间0.1 s,照射100个点)照射距离视盘1个视盘直径的静脉主干,眼底照相和荧光素眼底血管造影判断模型成功率。结果实验组内血小板黏附率及血小板聚集率明显降低,与对照组比较差异均有统计学意义(P<0.05);实验组内视网膜静脉完全阻塞的仅1例,而对照组有10例,差异有统计学意义(P<0.05)。结论丹参三七混合液口服可明显降低血小板黏附率,明显抑制血小板聚集,有预防视网膜静脉血栓形成的作用。
OBJECTIVE To observe the effect of the mixture liquor of salvia miltiorrhiza and Panax Notogin- seng （AP） in preventing retinal vein occlusion （RVO） on a rat model. METHODS Twenty male BN rats were ran- domized to receive AP （the intervension group, n=10） or physiological saline （the control group, n=10） for one month, and then, plasma of rats were collected to detect the platelet aggregation rate and platelet adhesion rate. Sub- sequently, photochemical thrombus formation was induced by intravenous injection of 30% Rose Bengal followed by Xenon laser of individual branch retinal veins of the eyes of 20 anesthetized BN rats. Complete occlusion was as- sessed by Fundus fluorescein angiography. RESULTS There were significant decreases of platelet aggregation rate and platelet adhesion rate in the intervension group and in next induced RVO study, of the 10 animals in the inter- vension group, 1 （10%） showed complete occlusion, whereas 10（100%） of the control group animals showed evident thrombosis. CONCLUSIONS The mixture liquor of AP had therapeutic benefit in preventing retinal lesions in the rat model of RVO.
出处
《中国中医眼科杂志》
2013年第2期88-90,共3页
China Journal of Chinese Ophthalmology
关键词
丹参
三七
视网膜静脉阻塞
血小板
salvia miltiorrhiza
panax notoginseng
retinal vein occlusion
platelet
