摘要
目的观察不同雄激素水平大鼠与大鼠海绵体神经型一氧化氮合酶（nNOS）和内皮型一氧化氮合酶（eNOS）表达的关系，探讨其临床意义。方法选取40只SD雄性大鼠，随机分为正常对照（NC）组10只，去势组30只，常规去势造模后分别于接受不同浓度的十一酸睾酮灌胃（20、10mg／kg）和生理盐水灌胃。4周后，采用免疫组织化学和Westernblot法检测各组阴茎海绵体组织中nNOS和eNOS的表达。结果NC组阴茎海绵体中nNOS和eNOS的表达量分别为98．24±3．02、80．04±2．96；1组的表达分别为92．81±3．37、78．75±4．02；2组的表达分别为45．70±3．83、75．68±5．36；3组的分别为20．24±4．06、71．64±3．04，结果显示去势组nNOS表达比NC组有明显的降低，在补充雄激素后恢复，而eNOS表达各组间差异无统计学意义（P〉0．05）。结论eNOS的表达是非雄激素依赖性的，而nNOS的表达是雄激素依赖性的，推测雄激素主要通过nNOS途径来调控阴茎平滑肌的舒张。
Objective To investigate the effect of different androgen levels on neuronal nitric ox- ide synthase （nNOS） and endothelial nitric oxide synthase （eNOS） in corpus cavernosum of castrated rats. Methods Forty Sprague-Dawley male rats were randomly divided into sham operated group （n=10） and operated group （ n = 30）. The rats in operated group were castrated and divided into 3 subgroups ： sub- groups 1 and 2 were treated with high dose （20 mg/kg） or low dose （10 mg/kg） testosterone undecanoate respectively each day, and subgroup group 3 was given normal saline. After 4 weeks of treatment, the rats in each group were sacrificed for detection of nNOS and eNOS by immunohistochemistry and Western blot- ting. Results nNOS and eNOS protein expression was detectable in the penis tissue of all rats. The nNOS and eNOS expressin levels in sham operated group were 98.24 ±3.02 and 80. 04±2. 96, 92. 81 ± 3.37 and 78.75±4. 02 in subgroup 1,45.70 ±3.83 and 75.68± 5.36 in subgroup 2, and 20. 24±4. 06 and 71.64 ±3.04 in subgroup 3. The nNOS expression level in castrated groups was lower than in sham opera- ted group and restored after testosterone supplement. There was no significant difference in the eNOS ex- pression among the groups. Conclusion The expression of eNOS is androgen independent, and that of nNOS is androgen dependent. Androgen regulates penile relaxation of smooth muscle probably through nNOS pathway.
出处
《中华实验外科杂志》
CAS
CSCD
北大核心
2013年第6期1195-1196,共2页
Chinese Journal of Experimental Surgery
