摘要
本研究构建了含有耐草甘膦基因G2-epsps和R79-epsps的双价植物表达载体pCMG2R79-EPSPS,以优良玉米自交系78599和综31的幼胚为受体材料,采用农杆菌介导法将耐草甘膦双价基因G2R79-epsps转入玉米幼胚细胞,以期获得耐草甘膦性更高的转基因玉米植株。目前已获得转基因再生玉米植株32株,经特异PCR检测表明其中7株植株转入了目标基因,阳性率达到21.9%。对得到的阳性植株的T1、T2代进行了跟踪检测,经田间喷施除草剂试验结果表明,获得了可耐草甘膦6‰的玉米材料,即生产上草甘膦田间使用浓度3倍水平的材料,为抗除草剂玉米新品种选育提供了较好的基础材料。
A bivalent ptant expression vector pCMG2R79-EPSPS expressingthe glyphosatetolerant G2-epsps gene and R79-epsps gene was constructed and transformed into the immature embryos of the elite maize lines 78599 and Zong31, for gaining more higher glyphosate-tolerance oftransgenic maize plants. To this day, 32 regeneration plants was acquired and the specific PCR detection showed that 7 plants of them were transferred with the target gene. The positive rate was 21.9%. Further analysis was performed on the T1 and T2 generation and it was,obtained 6%0 concentration glyphosate-resistant plants, that was 3 times concentration levels than the glyphosate commer- cial using did, by spraying herbicide glyphosate in field. It would provide a kind of better parent materials for breeding the new resistant herbicide glyphosate maize varieties.
出处
《分子植物育种》
CAS
CSCD
北大核心
2013年第3期339-344,共6页
Molecular Plant Breeding
基金
转基因生物新品种培育重大专项(2009ZX08003-003B)
四川省农科院财政基因工程项目(2011JYGC01-002)
农业部西部之光人才培养计划(2010-2011)
四川省农科院高新技术研究应用专项(2013GXJS-001)共同资助
关键词
玉米幼胚
双价基因G2R79-epsps
遗传转化
草甘膦抗性
农杆菌介导
Keywords Maize immature embryo, Bivalent gene G2R79-epsps, Transformation, Glyphosate-resistant, Agroba-cterium tumefaciens-mediated
