摘要
以经紫外线灭活的真鲷(Pagrosomus major)精子作为异源精子,诱导牙鲆(Paralichthys olivaceus)卵子进行有丝分裂雌核发育,通过静水压方法获得165个双单倍体作为实验材料。测量牙鲆身体纵轴6个生长相关性状(体长、头长、背鳍基长、腹鳍基长、尾柄长和尾长),并用SPSS 19.0对其进行正态分布及相关性分析。运用MapQTL4.0中多座位QTL模型(MQM)对控制6个性状的QTL进行定位及遗传效应分析。结果表明,所检测6个性状均符合正态分布,并且相互之间呈极显著相关(P<0.01)。在全部24个连锁群上检测到22个控制相关性状的QTL,分布在10个连锁群上。其中与体长相关的QTL有4个,头长相关的QTL为3个,控制背鳍基长的QTL为4个,与腹鳍基长相关的QTL为3个,与尾柄长相关QTL 7个,控制尾长的QTL仅有1个。各QTLs的LOD值在2.01-3.68之间,可解释3.7%-12.6%的表型变异。控制体长和尾长的QTL存在共定位,控制体长的QTL还与控制背鳍基长及腹鳍基长的QTL存在共定位。本研究结果可为今后进一步辅助育种和改良性状提供参考。
We obtained a double haploid population of Japanese flounder （Paralichthysolivaceus） by applying hy- drostatic pressure to the eggs of Japanese flounder following fertilization with UV-treated sperm from red sea bream （used as a mapping family）. We measured the size of six component elements of the cephalocaudal axis, including body length, head length, dorsal fin length, pelvic fin length, caudal peduncle length, and caudal length. The values for these six traits were all normally distributed and were significantly correlated （P〈0.01）.We docu- mented 22 quantitative trait loci （QTL） controlling the six growth-associated traitsusing MapQTL4.0. These QTLs were mapped on 10 of 24 linkage groups. Among these, four QTLs were correlated to body length, three to head length, four to dorsal fin length, three to pelvic fin length, seven to caudal peduncle length, and one to caudal length. The LOD values of these QTLs varied between 2.01 and 3.68, which explained 3.7%-12.6% of the pheno- typic variation. QTLs for body length and caudal length were co-localized; and QTLs for body length, dorsal fin length, and pelvic fin length were also co-localized. Our results provide insight for further selective breeding re- search and breed improvement.
出处
《中国水产科学》
CAS
CSCD
北大核心
2013年第3期514-520,共7页
Journal of Fishery Sciences of China
基金
国家公益性农业行业专项资助项目(200903046)
国家鲆鲽类产业技术体系资助项目(CARS-50-G2)
