摘要
目的:建立一种能够快速、准确地检测流感病毒亚单位疫苗中间体样品中沙门菌污染的方法。方法:首先利用选择性增菌培养基对样品进行增菌培养,然后提取样品中的细菌基因组DNA,通过沙门菌特异性引物对基因组DNA进行PCR扩增,以琼脂糖凝胶电泳实现对目的片段的检测。结果:该PCR反应体系的扩增检测灵敏度可达1 pg的沙门菌DNA,利用选择性增菌培养配合该PCR体系可在最快24 h内实现对沙门菌的准确检测,测定结果与传统方法相符。结论:此方法应用于流感病毒亚单位疫苗中间体的沙门菌检查,较之传统的培养法结合生化鉴定的方法,大大缩短了检测周期,降低了结果判读的难度,在实际生产中有很好的应用前景。
Objective： To establish a rapid and accurate detection for Salmonella in influenza virus subunit vaccine intermediates. Methods： Sample genomic DNA was extracted after cultivation in enrichment media, target segment was amplified by specific primers thereafter, and detected by agarose gel electrophoresis. Results： Sensitivity of the PCR assay was 1 pg Salmonella DNA. Detection assay could be accomplished properly in 24 hours by selective enrichment cultures and PCR, the results was in accord with traditional method. Conclusion： This method provided a faster and unambiguous resolution in Salmonella detection of influenza subunit vaccine intermediate, in comparison of traditional method of cultivation accompanied by biochemical assay, and has good application prospects in vaccine production.
出处
《生物技术通讯》
CAS
2013年第3期406-408,共3页
Letters in Biotechnology
