摘要
目的:研究水红花子黄酮类成分对人肝癌细胞株SMMC-7721增殖、细胞周期及细胞凋亡的影响。方法:采用MTT法研究水红花子黄酮类成分对人肝癌细胞SMMC-7721的抑制作用及时效量效关系;并通过PI染色法和Annexin V-EGFP/PI双染法观察水红花子黄酮类成分作用于人肝癌细胞株SMMC-7721后细胞周期DNA含量变化及细胞凋亡率。结果:水红花子黄酮类成分能调节SMMC-7721人肝癌细胞株G1/S转换,使肿瘤细胞发生S期阻滞,造成S期细胞堆积,阻断细胞的DNA合成和复制,阻滞肿瘤细胞进入G2/M期,从而抑制肿瘤细胞的增殖,诱导SMMC-7721细胞凋亡,且具有明显的时效及量效关系。结论:水红花子黄酮类成分对人肝癌细胞株SMMC-7721具有明显的抑制作用,且抑制作用和时间剂量成线性关系,其作用机制为抑制肿瘤细胞的增殖和诱导该细胞的凋亡。
Objective：To investigate the inhibitory effects of flavonoids of Polygoni Orientails Fructus on cell cycle and apoptosis of human hepatoma cell line SMMC-7721.Methods：MTT method was used to study the inhibitory rate and time-dose relationship of flavonoids on SMMC-7721.Flow cytometry PI staining and Annexin V-EGFP/PI double staining were used to measure the DNA concentration and apoptosis rate in SMMC-7721.Results：Flavonoids of Polygoni Orientails Fructus had obvious inhibitory effect and with good linear relationship between time and dose.Flavonoids of Polygoni Orientails Fructus could regulate the G1/S transition in hepatoma cell,which could make the cell in S phase arrest,result in the accumulation of cells in S phase,blocked DNA synthesis and replication of cells,blocked tumor cells into the G2/M phase,so as to restrain the proliferation of tumor cells and induce the apoptosis of SMMC-7721 cells.And this inductive effect showed obvious time-dose-effect relationship.Conclusion：The flavonoids of Polygoni Orientails Fructus could inhibit the hepatoma cell line SMMC-7721 in vitro by inhibiting cell proliferation and inducing apoptosis of the cell in a time-dose dependence maner.
出处
《中药材》
CAS
CSCD
北大核心
2013年第2期255-259,共5页
Journal of Chinese Medicinal Materials
基金
国家"重大新药创制"科技重大专项(2009ZX09103-354)
