摘要
本文采用高效液相色谱-二极管阵列检测器法(HPLC-DAD)快速检测杏中水溶性有机酸(苹果酸,柠檬酸和琥珀酸)和维生素(维生素C、维生素B1、维生素B2)含量。以0.5 mmol/L磷酸-乙腈为流动相,随着时间推移改变流速和流动相比例,13 min内梯度洗脱,采用多检测波长210 nm,254 nm和270 nm条件下,可使所测有机酸和维生素实现快速较好地分离。方法的稳定性在0.75-2.12%范围内,精密度在0.94-1.55%之间,回收率测定结果为96.7-101.9%。采用本方法测定不同地域杏样品,提出巨鹿杏的特征性品质。
High performance liquid chromatography （HPLC） with diode array detector （DAD） was applied to determine water-soluble organic acids （malic acid, citric acid and succinic acid） and vitamins （Vc, VB1 and VB2） in apricots sampled from Julu. The apricots samples were elulexi using 0.5 mmol/L HaPO4-acetonitrile as mobile phase at several wavelengths （210 nm, 254 nm and 270 nm）. The substances can be efficiently separated in 13 min with RSD value ranged in 0.75-2.12%. The precisions were between 0.94% and 1.55% and the result of recoveries was of 96.70-101.9%. The difference in organic acid and vitamin contents between Julu apricot and those sampled from other sources were also compared.
出处
《现代食品科技》
EI
CAS
北大核心
2013年第5期1123-1127,共5页
Modern Food Science and Technology
基金
河北省科学技术研究与发展计划项目(12221003D)
关键词
多波长
有机酸
维生素
巨鹿杏
multi-wavelengh
organic acid
vitamin
Ju＇lu Apricot
