摘要
本文研究了酶量、温度、pH和时间四个因素对黑蚂蚁蛋白的碱性酶法水解的影响。通过响应面设计,以各个抗氧化活性为指标,优化了超氧自由基清除率,DPPH自由基清除率,羟基自由基清除率,还原力和亚油酸自氧化抑制作用最强的酶解工艺条件。验证实验证实方程的准确率达95%以上。采用超滤膜及离子交换色谱对酶解产物进行了分离,发现5-10 kDa和1-3 kDa组分的抗氧化能力最强,碱性肽对超氧自由基清除能力、羟基自由基清除能力、亚油酸自氧化抑制作用贡献较大,酸性肽对DPPH自由基清除能力及还原力的贡献较大。
The influences of hydrolysis time, pH, temperature, enzyme and time on enzymatic hydrolysis of Polyrhachis vicina Roger protein by alcalase 2.4L were investigated. The antioxidant activity of the product was evaluated by determining five indexes including the scavenging activities of superoxide radical, DPpHo and hydroxyl radical, reducing power and inhibitory ability against oxidation of linoleic acid. By response surface methodology （RSM）, the hydrolysis conditions were optimized to achieve the highest antioxidant activity of the product. Verification experiments confirmed the accuracy of equations more than 95%. Ultrafillration and chromatography techniques were performed to purify enzymatic hydrolysates. It was found that peptides in the range of 5-10 kDa and 1-3 kDa mainly accounted for the activity. Basic peptides had greater scavenging capacity against superoxide radical and hydroxyl radical, as well as inhibitory ability against oxidation of linoleic acid, than acidic peptides. Acidic peptides had higher DPPH. radical scavenging activities and reducing power compared with basic peptides.
出处
《现代食品科技》
EI
CAS
北大核心
2013年第5期1089-1092,992,共5页
Modern Food Science and Technology
关键词
拟黑多刺蚁
酶解
抗氧化活性
膜分离
polyrhachis vicina roger
enzymatic hydrolysis
antioxidant activity
membrane separation
