摘要
目的:研究缬沙坦(Val)合并阿托伐他汀(Ato)对心肾综合征(CRS)大鼠心肾功能的影响及机制。方法:SD大鼠经"两次手术切除法"行3/4肾切除,1周后皮下注射异丙肾(100 mg/kg,分2次,间隔24 h),4周后将存活大鼠随机分为对照组(Control组)、Val组、Ato组和Val+Ato组,试剂盒法测定血清肌酐(Cr)、尿素氮(BUN)、B型尿钠肽(BNP)、醛固酮(ALD)、C-反应蛋白(CRP),Bradford法测定24 h尿蛋白量。治疗组给予Ator或(和)Val[10 mg/(kg.d)、80 mg/(kg.d)]、Control组给予生理盐水灌胃,干预4周后重新检测上述血清指标,左心室插管术测定左室收缩压(LVSP)、左室舒张压(LVDP)、左室舒张末压(LVEDP)及左室压力最大上升及下降速率(±dp/dtmax)。称重法测定心脏重量参数。切片HE染色观测心肌病理情况。结果:与Control组相比,各治疗组大鼠血清Cr、BUN、BNP、ALD、CRP、尿蛋白明显降低,LVSP上升,LVDP、LVEDP及心脏重量指数下降,差异有统计学差异。而Val+Ato组变化最显著。结论:缬沙坦与阿托伐他汀合用能更加有效保护CRS大鼠心肾功能。
Objective： To investigate the effects of combined treatment with Valsartan and Atorvastatin on chronic heart failure with renal failure in rats.Methods： Sprague-Dawley（SD） rats received three-quarters nephrectomy by twice resections,and after one week,the rats were subcutaneously injected with isoproterenol（100 mg/kg,twice injections,with a 24 hour interval）.Four weeks later,survival rats were randomly separated into four groups as control group,Valsartan group（Valsartan 80 mg/kg per day）,Atorvastatin group（Atorvastatin 10mg/kg per day）,and Val ＋ Ato group（Valsartan 80 mg/kg per day, Atorvastatin 10 mg/kg per day）.Serum creatinine（Cr）,blood urea nitrogen（BUN）,B-type natriuretic protein（BNP）,aldolase（ALD）,and C-reactive protein（CRP） were determined by kit assay.24 h urine protein was determined by the Bradford method.After Four weeks,the serum and urine protein were determined repeatedly,LVSP,LVDP,LVEDP,dp/dtmax and-dp/dtmax were determined by left ventricular intubation.Heart weight indices were determined and the myocardial pathological conditions were observed by hematoxylin and eosin（HE） staining.Results： Compared with the control group,each index of serum and urine protein in the treatment group was significantly decreased,Additionally,LVDP, LVEDP,and heart weight index were decreased,while LVSP and dp/dtmax were increased markedly（P 0.05）.Val ＋ Ato group showed more changes than single treatment group.Conclusion： Combination of Valsartan and Atorvastatin are more effective on chronic heart failure and renal failure in rats than Valsartan or Atorvastatin administrates alone.
出处
《武汉大学学报（医学版）》
CAS
北大核心
2013年第3期352-355,共4页
Medical Journal of Wuhan University
基金
武汉市卫生局研究资助项目(编号:WX11C18)
