摘要
目的比较两种不同组合方式所得粗提物的抗氧化活性,探讨不同总黄酮组分组合对抗氧化活性影响。方法分别采用先提取后组合和药材混合提取的方式制备总黄酮,采用比色法测定总黄酮含量,以Fenton反应-比色法,测定羟自由基清除率。结果在总黄酮量相同的情况下,提取后组合的羟自由基清除效果优于混合提取。结论中药有效部位组合可以体现中药的协同作用。
Objective To compare the antioxidant activity of two extracts with different ways, and discuss the influence of different flavonoids combination to the antioxidant activity. Methods Total flavonoids were prepared using a combination of extracted and mixed extracted, and contents of total flavonoids were determined by spectrophotometric method, the hydroxyl radical scavenging was measured with the reaction of Fenton and spectrophotometric method. Results With the same content of total flavonoids, hydroxyl radical scavenging effect of combination after extraction was better. Conclusion The combination of active components from traditional Chinese medicine could reflect the synergistic effect.
出处
《食品与药品》
CAS
2013年第3期160-161,共2页
Food and Drug
关键词
桑叶
鬼针草
组分中药
抗氧化
leaf of Morus alba L.
Bidens bipinnata L.
multi-component Chinese medicine
antioxidant.
