摘要
目的:研究重组人肿瘤坏死因子受体融合蛋白(rhTNFR:Fc)对佐剂性关节炎(adjuvant arthri-tis,AA)大鼠滑膜细胞增殖和分泌功能的影响。方法:完全弗氏佐剂诱导SD大鼠AA模型,3H-TdR掺入法检测滑膜细胞增殖,ELISA法测定滑膜细胞上清中白细胞介素1β(IL-1β),IL-6,IL-17,淋巴细胞毒素(LTα),NF-κB配体的受体或激活因子(RANKL),基质金属蛋白酶-13(MMP-13)水平。结果:rhTNFR:Fc体外给药可以抑制TNF诱导的滑膜细胞增殖,下调IL-1β,IL-6,IL-17,LTα,RANKL,MMP-13的分泌水平。结论:rhTNFR:Fc抑制滑膜细胞增殖和分泌功能可能是其治疗AA的作用机制之一。
Objective： To investigate the effect of rhTNFR： Fc on proliferation and cytokines production of synoviocytes from adjuvant arthritis （AA） rats. Methods： AA rats were induced by complete Freund adjuvant. The proliferation of synoviocytes was detected by 3H-TdR. The levels of IL-113, IL-6, IL-17, LTα, RANKL and MMP-13 in supernate of synoviocytes were assessed by ELISA. Results： Administration of rhTNFR： Fc significantly inhibited the proliferation of synoviocytes and decreased the secretion of IL-lβ, IL-6, IL-17, LTα, RANKL and MMP-13. Conclusions： Our results indicate that rhTNFR：Fc significantly reduces the proliferation and proinflamma- tory cytokines production of synoviocytes, which might be one of the therapeutic mechanisms for rhTNFR：Fc in AA.
出处
《中国新药杂志》
CAS
CSCD
北大核心
2013年第10期1166-1169,共4页
Chinese Journal of New Drugs
基金
国家自然科学基金(30973543
31100640
81173075)
安徽省高校自然科学基金(KJ2011Z180
KJ2011Z181)
