摘要
目的研究小剂量阿司匹林对糖尿病大鼠肾脏的保护作用,并探讨可能的机制。方法雄性SD大鼠,尾静脉注射链脲佐菌素30 mg/kg,造成1型糖尿病动物模型,随机分为糖尿病模型组和9 mg/kg阿司匹林治疗组,每组8只,另选8只健康雄性大鼠为正常对照组。各组大鼠灌服给药12周后,检测各组大鼠空腹血糖、血尿素和肌酐,以及尿蛋白含量,HE染色法检查肾组织病理显微结构,免疫组织化学法检测分析Caspase-3、Bax和Bcl-2的表达情况。结果与正常对照组比较,糖尿病模型组大鼠空腹血糖、血尿素和肌酐,以及尿蛋白含量均升高,肾小球萎缩明显,肾组织Caspase-3、Bax表达增强,肾组织Bcl-2的表达减弱,Bcl-2/Bax比值降低;阿司匹林治疗组大鼠上述指标均有改善。结论小剂量阿司匹林对糖尿病大鼠肾脏有保护作用,可能与调节细胞凋亡相关蛋白表达有关。
Aim To investigate the effect of low-dose aspirin on kidney in diabetic rats and discuss possible mechanism. Methods Male SD rats were injected streptozotocin （STZ） 30 mg/kg by tail intravenous for establishing the type 1 diabetes animal model, which were randomly divided into diabetes model group and 9 mg/kg aspirin i. e. acetyl- salicylic acid （ASA） treatment group, and other 8 healthy male SD rats were included into the control group. The rats took medicine by irrigation stomach for 12 weeks, then their fasting blood glucose, blood urea and serum creatinine, and ufine protein content were inspected, kidney tissue pathology microstructure was inspected by HE dyeing method, and Caspase-3, Bax and Bcl-2 expression was detected by immunohistochemical method. Results Compared with the normal control group rats, in diabetes model rats, the content of fasting blood glucose, blood urea, serum creatinine and urine protein was increased, glomerular atrophy was more obviously, kidney tissues Caspase-3 and Bax express were enhanced, meanwhile kidney tissues Bcl-2 expression and Bcl-2/Bax ratio were decreased; in ASA treatment rats, the above indexes were improved. Conclusion Low-dose aspirin has protective effect in diabetic rats kidney, which may regulate the ex- pression of cell apoptosis related protein.
出处
《中国动脉硬化杂志》
CAS
CSCD
北大核心
2013年第5期419-423,共5页
Chinese Journal of Arteriosclerosis
