摘要
了解谷胱甘肽（ＧＳＨ）对高糖时人内皮细胞分泌前列环素（ＰＧＩ２）和内皮素－１（ＥＴ－１）的影响。方法： 原代培养人脐静脉内皮细胞，分别加入正常对照组，高糖组，正常＋ＧＳＨ组，高糖＋ＧＳＨ组培养基，放免法测定培养 ２４ｈ，４８ｈ，７２ｈ时培养基中的ＰＧＩ２和ＥＴ－１含量。结果：①高糖组较正常对照组ＰＧＩ２显著下降（Ｐ＜０．０１），ＥＴ－１显 著升高（Ｐ＜０．０１）；②高糖＋ＧＳＨ组较高糖组ＰＧＩ２升高（Ｐ＜０．０１），ＥＴ－１显著下降（Ｐ＜０．０１）。结论：ＧＳＨ在调节高 糖时人血管内皮细胞分泌 ＰＧＩ２／ＥＴ－１的平衡方面起一定作用。
Aim: To investigate the influence of reduced glutathion(GSH) on prostacyclin (PGI2) and endothelin-1(ET-1) secretion of human umbilical vein endothelial cells (HUVEC) cultured with a high concentration of glucose. Methods: The medi- um PGI2 and ET-1 were assayed by RIA in HUVEC which were cultured at 24,48 and 72 hours. Results: ①the content of PGI2 in the high glucose group was lower than that in normal contrast group (P < 0.01), and the content of ET-1 was higher (P < 0.01); ②the content of PGI2 in the high glucose with reduced glutathione group was higher than that in the high glucose group (P <0.05), and the content of ET-1 was lower (P<0.01). Conclusion: GSH can balance the secretion of PGI2/ET-1 by HU- VEC.
出处
《河南医科大学学报》
2000年第5期431-433,共3页
Journal of Henan Medical University
