摘要
目的:探讨高糖对人肾小球系膜细胞细胞间黏附分子1(ICAM-1)表达的影响及茶多酚对其干预作用。方法:将培养的系膜细胞分为正常对照组、高糖组、茶多酚干预组和茶多酚对照组,分别在0、12、36h采用RT-PCR法和免疫细胞化学法检测茶多酚对高糖条件下系膜细胞内ICAM-1mRNA及蛋白表达的影响。结果:高糖组系膜细胞ICAM-1mRNA和蛋白的表达比正常对照组均增加(P<0.05);茶多酚干预组比高糖组有明显下降(P<0.05)。结论:高糖可导致人肾小球系膜细胞ICAM-1表达的增加,而茶多酚可有效干预此效应。
Objective：To investigate the effects of high glucose on the expression of ICAM-lmRNA in human mesangial cells and the intervention of tea polyphenols （TPs）. Methods：Human mesangial cells were cultured in DMEM media containing high glucose with or without TPs for 0,12,36 hours,respectively, The mRNA and protein expression of ICAM-1 were measured by RT-PCR and immunocytochemistry. Results：The mRNA and protein expression of ICAM-1 of the cells increased significantly in high glucose medium （P 〈 0.05）. However,TPs inhibited the expression （P 〈 0.05）. Conclusion：High glucose contributes to the increase of ICAM-1 expression in human mesangial cells. TPs can reduce high glucose-induced expression of ICAM-1.
出处
《南京医科大学学报（自然科学版）》
CAS
CSCD
北大核心
2007年第4期295-298,F0002,共5页
Journal of Nanjing Medical University(Natural Sciences)
基金
国家自然科学基金资助项目(30370658)
