摘要
实验测量了在405 nm的脉冲激光激发下,人体离体正常和癌变结肠组织在635 nm荧光发射峰的时间分辨自体荧光光谱特性。采用双指数衰减方程对时间分辨自体荧光光谱进行拟合,得到相应的平均荧光寿命。结果显示:人体正常和癌变结肠组织在405～635 nm激发发射波长条件下的自体荧光平均寿命分别为(4.87±0.75)ns和(13.27±3.31)ns,二者存在显著差异。采用时间分辨自体荧光光谱区分人体正常和癌变结肠组织的灵敏度和特异性分别为100%和93%。初步表明时间分辨自体荧光光谱有望用于诊断早期结肠癌。
Time-resolved autofluorescence spectroscopies at 635 nm under 405 nm excitation were measured for human normal and abnormal colonic tissues in vitro. The main fluorescence lifetime of the tissues were calculated by the two-component lifetime fitting of time-resolved autofluorescence spectroscopies. The results showed that the average lifetime of normal and abnormal human colonic tissues are about （4.87±0.75） ns and （13.27±3.31） ns, respectively. In this method, the sensitivity and specificity for diagnosis of human colonic carcinoma tissue are 100% and 93%. Experimental results indicated that the time-resolved autofluorescence spectroscopies can be potentially used to distinguish early human colonic carcinoma tissue.
出处
《三明学院学报》
2013年第2期49-52,共4页
Journal of Sanming University
基金
三明学院自然科学基金项目(B201009/Q)
关键词
时间分辨自体荧光光谱
荧光寿命
结肠组织
组织诊断
time-resolved autofluorescence spectroscopies
fluorescence lifetime
human colonic tissues
tissue diagnosis
