摘要
目的 了解急性期过敏性紫癜 (HSP)患儿是否存在淋巴细胞延迟凋亡及Bcl 2和CD40L对它们的影响。方法 分别用琼脂糖凝胶电泳及流式细胞技术 (FACS)分析经CD3单克隆抗体刺激培养的外周血单个核细胞 (PBMC)产生的凋亡片段DNA及凋亡细胞计数 ;FACS检测PBMCBcl 2的产生及CD40L表达。结果 与正常儿童比较 ,急性期HSP患儿的PBMC出现延迟凋亡现象 ,Bcl 2产生及CD40L表达增高 ,且Anti CD40L能明显纠正延迟凋亡。结论 HSP患儿的淋巴细胞及单核细胞存在延迟凋亡 ,可能与Bcl 2的异常产生及CD40L过度表达有关 。
Objective To observe whether lymphocytes apoptosis delays in children with Henoch Schnlein's purpura (HSP) in the acute phase and investigate the effects of Bcl 2 and CD40L on it. Methods DNA fragmentation and apoptosis cell count of peripheral blood mononuclear cells (PBMC) stimulated with anti CD3 McAb were detected by both agarose gel electrophoresis and flow cytometry. Analyses of Bcl 2 production and CD40L expression on PBMC were also conducted by flow cytometry. Results The results showed the apoptosis delay and significant increases of Bcl 2 production and CD40L expression of PBMC. This apoptosis delay condition of PBMC could be corrected when anti CD40L McAb was added in vitro. Conclusions CD40L and Bcl 2 might involve in regulating apoptosis of PBMC of the patients. High expression of CD40L and Bcl 2 might result in delayed apoptosis of lymphcytes, which might play a very important role in setup of HSP.
出处
《中华微生物学和免疫学杂志》
CAS
CSCD
北大核心
2000年第5期411-413,共3页
Chinese Journal of Microbiology and Immunology
