摘要
目的通过观察Z-藁本内酯对肿瘤坏死因子-α(TNF-α)诱导的人脐静脉内皮细胞(HUVEC)细胞间黏附分子-1(ICAM-1)、血管细胞黏附分子-1(VCAM-1)及核因子-κB(NF-κB)表达的影响,探讨其对炎性因子损伤内皮细胞的保护作用及其机制。方法向体外培养的HUVEC中加入TNF-α10 ng/mL造成细胞损伤,同时加入不同浓度(5、10、20μmol/L)的Z-藁本内酯,共同孵育24 h,MTT法检测各组细胞活力,ELISA法测定细胞培养液中ICAM-1和VCAM-1的量,Westernblotting法检测各组细胞NF-κB蛋白表达。结果与对照组比较,TNF-α可造成HUVEC明显损伤(P<0.05、0.01),显著增加HUVEC中ICAM-1和VCAM-1的表达(P<0.01)。与模型组比较,Z-藁本内酯可以剂量相关地降低ICAM-1(P<0.01)和VCAM-1(P<0.05)的表达,显著减少细胞NF-κB蛋白的表达(P<0.05)。结论 Z-藁本内酯可减轻TNF-α对HUVEC的损伤,其作用机制可能与通过抑制NF-κB的激活,进而减少ICAM-1和VCAM-1的表达有关。
Objective To observe the effect of Z-ligustilide on the expression of tumor necrosis factor-α （TNF-α）-induced human umbilical vein endothelial cells （HUVEC） intercellular adhesion molecule-1 （ICAM-1）, vascular cell adhesion molecule-1 （VCAM-1）, and nuclear factor kappa B （NF-κB）, and to investigate the protection on endothelial cells injured by inflammatory cytokines and its mechanism. Methods TNF-α （10 ng/mL） was added to in vitro cultured HUVEC to induce the cell injury. After co-incubated with the different concentration （5, 10, and 20 9μol/L） of Z-ligustilide for 24 h, the cell viability in each group was measured by MTT. The levels of ICAM-1 and VCAM-1 were assayed using enzyme linked immunosorbent assay （ELISA）. Western blotting was used to detect the expression of NF-κB protein of cell in each group. Results TNF-α could induce the obvious injury of HUVEC compared with the control group （P 〈 0.05, 0.01）, and increase the expression of ICAM-1 and VCAM-1 in HUVEC （P 〈 0.01）. Compared with the model group, Z-ligustilide could dose-dependently reduce the expression oflCAM-1 （P 〈 0.01） and VCAM- 1 （P 〈 0.05） and obviously decrease the NF-κB expression （P 〈 0.05）. Conclusion Z-ligustilide could attenuate TNF-α-induced injury in HUVEC via suppressing the activation of NF-κB and inhibiting the excessive expression of ICAM-1 and VCAM-1.
出处
《中草药》
CAS
CSCD
北大核心
2013年第9期1157-1161,共5页
Chinese Traditional and Herbal Drugs
基金
科技部国际合作课题(2009DFB30510)
