摘要
【目的】研究贵州地方山羊品种黔北麻羊、贵州白山羊和贵州黑山羊ras-related and estrogen-regulated growth inhibitor(RERG)基因第2、3和4外显子的多态性及其与生长性状的相关性。【方法】本试验通过PCR-SSCP技术和直接测序法,对3个品种外显子SNPs位点进行检测,利用一般线性模型分析其与生长性状的关联性。【结果】3个供试群体中在第4外显子上都检测到4个SNPs位点,即56 bp(C/G)、826 bp(A/G)、1 434 bp(T/C)和1 798 bp(A/T)。最小二乘法分析结果表明,AA型和BB型的体重对AB型达到差异极显著水平(P<0.01),CC型和DD型的体重对CD型达到差异显著水平(P<0.05),EE型和FF型的体重对EF型达到差异极显著水平(P<0.01)、1 798 bp(A/T)位点各基因型间差异不显著。【结论】RERG基因的56 bp(C/G)、826 bp(A/G)和1 434 bp(T/C)多态性位点可作为生长性状的候选分子标记。
[Objective] This study aimed at researching polymorphism of the second, third and fourth exons ofras-related and estrogen-regulated growth inhibitor （RERG） gene in three indigenous Guizhou goats （Qianbei Ma goat, Guizhou White goat and Guizhou Black goat） and its association with growth traits was analyzed. [Method] PCR-SSCP and PCR-direct sequencing were applied to detect the polymorphism sites and the general linear model was used to analyze its association with growth traits. [Result] Four consistent SNPs sites were found in the fourth exon of RERG gene in such three breeds. They are 56 bp C→G, 826 bp A→G, 1 434 bp T→C and 1 798 bp A→T. The following fixed effects model was employed in analyzing the growth traits in goat. The body height was significantly higher in individuals with genotype AB than AA and BB and genotype EF than EE and FF （P〈0.01）. A significant difference was found in body weight between genotype CD and CC and DD （P〈0.05）. The 1 798 bp （A/T） site did not appear significant difference. [ Conclusion] The 56 bp （C/G）, 826 bp （A/G） and 1 434 bp （T/C） sites will provide a study basis between RERG genes and growth traits as a well as certain theoretical foundation for better feeding and genetic resources of indigenous goats.
出处
《中国农业科学》
CAS
CSCD
北大核心
2013年第9期1915-1922,共8页
Scientia Agricultura Sinica
基金
贵州省农业科技攻关项目(黔科合NY字(2008)3044)
贵州大学研究生创新基金(农科2012036)
