摘要
目的探讨顺铂联合低分子柑橘果胶(LCP)对人肺癌细胞的增殖及凋亡通路的影响。方法采用四甲基偶氮唑盐法检测各浓度顺铂单药(单药组)、顺铂联合LCP(联合组)对人肺癌细胞A549增殖的影响,用流式细胞仪检测半抑制浓度顺铂单药及联合用药处理A549细胞的凋亡比例,采用Western blot分析凋亡相关蛋白procaspase-3、8、9的变化。结果顺铂单药与联合LCP用药对A549细胞生长均有抑制作用,其效应呈时间和浓度依赖性;联合组对A549细胞的生长抑制作用更为显著(P<0.01)。单药与联合用药均能使A549细胞凋亡比例增加,联合组较单药组细胞凋亡更加显著。单药组与联合组A549细胞的凋亡相关蛋白procaspase-3、8、9蛋白的表达均下调,联合组较单药组procaspase-3、9蛋白表达下调更为显著,但两组procaspase-8蛋白表达无明显差异。结论 LCP能增加顺铂的细胞增殖抑制和诱导凋亡能力,该效应可能与活化线粒体凋亡途径有关。
Objective To study the effects of DDP combined with low-molecular-weight citrus pectin（LCP） on proliferation and apoptosis of human lung carcinoma cell .Methods The effects of DDP with（DDP group） or without LCP（combination group） in different concentrations on the proliferation of human lung carcinoma cell line A 549 were determined by MTT assay ,and the cell apoptosis proportion of A549 cell treated with DDP,DDP and LCP（IC50） was analyzed by facial action coding system（FACS）.The changes of cell apoptosis-related proteins including procaspase-3,8,9 were measured with Western blot.Results DDP inhibited A549 cell proliferation in a time-and dose-dependent manner as well as DDP combined with LCP;The inhibitory effect of combination group on A549 cell proliferation was more significant（P〈0.01）.DDP group and combination group could increase the apoptosis proportion of A 549 cell,and the increase was more significant in the combination group .Expressions of cell apoptosis-related proteins including procaspase-3,8,9 all decreased in two groups,and the down-regulation of procaspase-3,9 protein expressions in the combination group was more significant than that in the DDP group,but procaspase-8 protein expression showed no significant difference between two groups.Conclusion LCP can enhance the ability of DDP to inhibit cell proliferation and induce cell apoptosis,which may be related to the activation of mitochondrial apoptosis pathway.
出处
《广西医学》
CAS
2013年第4期422-425,共4页
Guangxi Medical Journal
关键词
肺癌
顺铂
低分子柑橘果胶
细胞增殖
细胞凋亡
Lung cancer
DDP
Low-molecular-weight citrus pectin
Cell proliferation
Cell apoptosis
