摘要
目的探讨高机械指数诊断超声联合微泡对大鼠Walker-256肿瘤微循环的作用。方法将29只皮下荷Walker-256肿瘤SD大鼠随机分为超声微泡组(n=15)、单纯超声组(n=7)和假照组(n=7):对超声微泡组采用声辐射力脉冲(ARFI)成像模式下诊断超声连续激励20次辐照肿瘤,同时经尾静脉推注微泡0.04ml;对单纯超声组在行超声辐照的同时以等量生理盐水代替微泡;对假照组则采用假照方式,仅推注等量微泡溶液,但不发射超声能量。对所有动物于辐照前、辐照后即刻、10、20min行CEUS检查。最后每组随机选取3只动物获取肿瘤组织标本,行病理学检查。结果辐照后即刻,超声微泡组辐照区几乎无造影剂充填,呈负性显影,肿瘤区平均造影峰值强度(PI)由25.17%减低到12.01%(P<0.01);单纯超声组及假照组辐照后即刻可见造影剂快速充填,灌注良好(P>0.05)。10min后,超声微泡组造影可见血流逐渐恢复,但PI仍降低;20min后肿瘤血流基本完全恢复,呈高灌注(P>0.05)。结论高机械指数诊断超声联合微泡能特异性地暂时降低大鼠Walker-256皮下移植瘤的微循环。
Objective To investigate the effect of high mechanical index diagnostic ultrasound combined with microbub- bles on the neovasculature of Walker-256 tumor in rats. Methods Twenty-nine SD rats bearing subcutaneous Walker-256 tumor were randomly divided into 3 groups： Ultrasound＋microbubbles group （n= 15）, ultrasound group （n= 7） and the sham group （n= 7）. CEUS was performed to acquire the baseline tumor blood perfusion of all the tumors. During the pro- cedure, the tumors were insonated with 20 times of continuous acoustic radiation force impulse imaging. At the same time of ARFI performance, 0. 04 ml microbubbles was instantly injected into the tail vein. The ultrasound group and sham group were applied with only diagnostic ultrasound exposure or microbubbles injection, respectively. CEUS was repeated to ob- serve the changes of perfusion before insonation and 0, 10 and 20 min after insonation. Three rabbits were harvested ran- domly in every group for histological examination immediately after insonation, Results The mean peak intensity （PI） values of ROI displayed significant decreases from 25.17 % to 12.01% in ultrasound＋microbubbles group （P〈0.01）. In the ultrasound group and sham group, media was filling quickly and perfusion was good. In ultrasound＋microbubbles group, the blood flow recovered gradually, but PI still reduced after 10 min, the blood flow of tumor recovered basically complete and showed high perfusion phase after 20 min （P〉0. 05）. Conclusion Microbubbles enhanced high mechanical index diagnostic ultrasound can temporally decrease the microcirculation of Walker-256 tumor in rats.
出处
《中国医学影像技术》
CSCD
北大核心
2013年第5期688-692,共5页
Chinese Journal of Medical Imaging Technology
基金
成都军区总医院院管课题(424121AH)
关键词
超声检查
微泡
造影剂
肿瘤
微循环
Ultrasonography
Microbubbles
Contrast media
Neoplasms
Microcirculation
